Y-specific primers
| Y-Specific Gene . | Sequence (5′-3′) . |
|---|---|
| DBY | Forward: CGCTTGAATTCAAGGATGAGTCATGTGGTG |
| Reverse: GGGTGATATCGCTGCAAAGCAGATTCAGTTG | |
| DFFRY 1 | Forward: AGATCTAAGCTTTGTCAAGCATGACAGCCATC |
| Reverse: TCCTAATTAATATCAGGATCCAAGTCTGGTTC | |
| DFFRY 2 | Forward: GCAAAACAAATAAGGATGTGCTTAGCAGAA |
| Reverse: AATTAATGAGGATATCCAAATACATCATCTCG | |
| DFFRY 3 | Forward: CGACATGTTCGGGGATGAGAAGCAGGACAG |
| Reverse: CATAGCGGCCGCAGTTAATTATTGCTTTTCAC | |
| EIF-1AY | Forward: TCACCGCCATGCCCAAGAATAAAGG |
| Reverse: CACTTGGTTCAATTTAGATATCATCAATG | |
| RPS4Y | Forward: CAGAATTCCGCCATGGCCCGGGGC |
| Reverse: AAAAAAAGATATCCTGCTACTGCAATTTAG | |
| SMCY 1 | Adaptor primer: CCATCCTAATACGACTCACTATAGGGC |
| Reverse: TCAATGTACAGGCATGTTGAAGTAGTCGGAC | |
| SMCY 2 | Forward: CACCATGGCTACCCAGGAGTACAGTTTGC |
| Reverse: TCAGGAGGCTATCTTGGCACCCATA | |
| SMCY 3 | Forward: CACCATGGCCCTGGCCCCTTCTGCTCAC |
| Adaptor primer: CCATCCTAATACGACTCACTATAGGGC | |
| TB4Y | Forward: CGCAGCCATGTCTGACAAACCTGG |
| Reverse: GATATTGGTGGCACATGCCTGTTTAA | |
| UTY | Forward: AATTTGTTGGTACCATGAAATCCTGCGC |
| Reverse: AATACTCGAGGAACTATATCAAGATGAGGA | |
| ZFY | Forward: GACCATGGATGAAGATGAATTTGAATTGCAG |
| Reverse: CAAGCTCGAGGACACACTGTTAGGGCAGA |
| Y-Specific Gene . | Sequence (5′-3′) . |
|---|---|
| DBY | Forward: CGCTTGAATTCAAGGATGAGTCATGTGGTG |
| Reverse: GGGTGATATCGCTGCAAAGCAGATTCAGTTG | |
| DFFRY 1 | Forward: AGATCTAAGCTTTGTCAAGCATGACAGCCATC |
| Reverse: TCCTAATTAATATCAGGATCCAAGTCTGGTTC | |
| DFFRY 2 | Forward: GCAAAACAAATAAGGATGTGCTTAGCAGAA |
| Reverse: AATTAATGAGGATATCCAAATACATCATCTCG | |
| DFFRY 3 | Forward: CGACATGTTCGGGGATGAGAAGCAGGACAG |
| Reverse: CATAGCGGCCGCAGTTAATTATTGCTTTTCAC | |
| EIF-1AY | Forward: TCACCGCCATGCCCAAGAATAAAGG |
| Reverse: CACTTGGTTCAATTTAGATATCATCAATG | |
| RPS4Y | Forward: CAGAATTCCGCCATGGCCCGGGGC |
| Reverse: AAAAAAAGATATCCTGCTACTGCAATTTAG | |
| SMCY 1 | Adaptor primer: CCATCCTAATACGACTCACTATAGGGC |
| Reverse: TCAATGTACAGGCATGTTGAAGTAGTCGGAC | |
| SMCY 2 | Forward: CACCATGGCTACCCAGGAGTACAGTTTGC |
| Reverse: TCAGGAGGCTATCTTGGCACCCATA | |
| SMCY 3 | Forward: CACCATGGCCCTGGCCCCTTCTGCTCAC |
| Adaptor primer: CCATCCTAATACGACTCACTATAGGGC | |
| TB4Y | Forward: CGCAGCCATGTCTGACAAACCTGG |
| Reverse: GATATTGGTGGCACATGCCTGTTTAA | |
| UTY | Forward: AATTTGTTGGTACCATGAAATCCTGCGC |
| Reverse: AATACTCGAGGAACTATATCAAGATGAGGA | |
| ZFY | Forward: GACCATGGATGAAGATGAATTTGAATTGCAG |
| Reverse: CAAGCTCGAGGACACACTGTTAGGGCAGA |