Summary of histopathology findings in mouse toxicity studies
| Test Group . | 3 Days postinjection . | 14 Days postinjection . | ||||||
|---|---|---|---|---|---|---|---|---|
| A . | B . | C . | D . | A . | B . | C . | D . | |
| Heart | N | N | N | N | N | N | N | N |
| Lung | N | N | N | N | N | N | N | N |
| Liver | ||||||||
| Inflammation/necrosis | 0.25 | 0.17 | 0.08 | 0.25 | 0.5 | 0.25 | 0.25 | 0.25 |
| Spleen | ||||||||
| Extramedullary hematopoiesis | 2.0 | 2.0 | 1.67 | N | 2.0 | 2.0 | 0.33 | N |
| Apoptosis (white pulp) | 0.5 | 0.5 | 0.5 | 0.25 | 0.5 | 0.5 | 0.42 | N |
| Kidneys | ||||||||
| Degeneration/necrosis | 0.75 | 0.5 | 1.33 | 0.63 | 0.88 | 0.58 | 0.67 | N |
| Inflammation | 1.00 | N | N | N | N | N | 0.08 | N |
| Test Group . | 3 Days postinjection . | 14 Days postinjection . | ||||||
|---|---|---|---|---|---|---|---|---|
| A . | B . | C . | D . | A . | B . | C . | D . | |
| Heart | N | N | N | N | N | N | N | N |
| Lung | N | N | N | N | N | N | N | N |
| Liver | ||||||||
| Inflammation/necrosis | 0.25 | 0.17 | 0.08 | 0.25 | 0.5 | 0.25 | 0.25 | 0.25 |
| Spleen | ||||||||
| Extramedullary hematopoiesis | 2.0 | 2.0 | 1.67 | N | 2.0 | 2.0 | 0.33 | N |
| Apoptosis (white pulp) | 0.5 | 0.5 | 0.5 | 0.25 | 0.5 | 0.5 | 0.42 | N |
| Kidneys | ||||||||
| Degeneration/necrosis | 0.75 | 0.5 | 1.33 | 0.63 | 0.88 | 0.58 | 0.67 | N |
| Inflammation | 1.00 | N | N | N | N | N | 0.08 | N |
Dosages: group A (3 × 1011vp), group B (1 × 1011vp), group C (2.5 × 1011vp), and group D (vehicle). Six mice per group were injected through the tail vein with the indicated doses. Tissue sampling: three mice from each group were killed on PI days 3 and 14, and tissues were processed for histopathology evaluation. Severity grading: 1, minimal; 2, mild; 3, moderate; 4, marked. Grades were determined by the average number for all animals examined and a correction factor for lesion appearance (focal, multifocal, or locally extensive). N, no change.