RAR antagonist effects on cell number and CFC output of cultured hematopoietic precursors
| AGN 193109 . | Cell Counts . | CFU-GM . | HPP-Mix . | |||
|---|---|---|---|---|---|---|
| Experiment 1 . | Experiment 2 . | Experiment 1 . | Experiment 2 . | Experiment 1 . | Experiment 2 . | |
| − | 0.77 × 106 | 1.18 × 106 | 6000 ± 764 | 4000 ± 1155 | 2333 ± 1202 | 523 ± 333 |
| + | 0.77 × 106 | 1.30 × 106 | 6750 ± 661 | 6333 ± 882 | 6333 ± 667 | 917 ± 300 |
| AGN 193109 . | Cell Counts . | CFU-GM . | HPP-Mix . | |||
|---|---|---|---|---|---|---|
| Experiment 1 . | Experiment 2 . | Experiment 1 . | Experiment 2 . | Experiment 1 . | Experiment 2 . | |
| − | 0.77 × 106 | 1.18 × 106 | 6000 ± 764 | 4000 ± 1155 | 2333 ± 1202 | 523 ± 333 |
| + | 0.77 × 106 | 1.30 × 106 | 6750 ± 661 | 6333 ± 882 | 6333 ± 667 | 917 ± 300 |
Counts and colony-forming cells (CFU-GM and HPP-mix) are estimated to be from all cells that grew in culture from 1000 lin− c-kit+ Sca-1+ cells after 7 days in liquid suspension culture, and are given as the total cell number and colony-forming cells (mean ± SEM, n = 3). The culture medium was supplemented with 100 ng/mL SCF, IL-6, Flt-3L, and 10 ng/mL IL-11, without (−) or with (+) 1 μmol RAR antagonist (AGN 193109).