Percentage of donor cells in myeloid and lymphoid lineages
| . | Summary Data5-150 . | |||||
|---|---|---|---|---|---|---|
| I. Short-term Reconstitution Analysis . | Percentage of Donor Cells in . | |||||
| Experiment # . | Months Post-BMT . | Culture Conditions . | Gr-1 . | CD11b . | B220 . | Thy-1.2 . |
| 1 | 3 | Fresh precursors | 36.3 ± 3.84 | 41.5 ± 4.39 | 51.0 ± 6.44 | 32.6 ± 5.20 |
| 1 | 3 | No ATRA D7 | 23.5 ± 7.72 | 23.8 ± 8.08 | 32.1 ± 9.89 | 25.5 ± 8.32 |
| 1 | 3 | ATRA D7 | 20.4 ± 5.85 | 22.1 ± 7.63 | 33.9 ± 6.38 | 24.9 ± 5.69 |
| 1 | 3 | No ATRA D14 | ND | ND | ND | ND |
| 1 | 3 | ATRA D14 | 8.77 ± 1.03 | 6.68 ± 1.26 | 28.6 ± 4.06 | 10.5 ± 2.59 |
| 2 | 3 | Fresh precursors | 43.5 ± 3.25 | 49.9 ± 6.13 | 54.2 ± 4.43 | 43.0 ± 3.78 |
| 2 | 3 | No ATRA D7 | 19.9 ± 5.08 | 20.0 ± 8.24 | 34.3 ± 7.43 | 20.9 ± 4.26 |
| 2 | 3 | ATRA D7 | 35.5 ± 6.29 | 30.0 ± 9.66 | 51.7 ± 6.81 | 32.5 ± 5.55 |
| 2 | 3 | No ATRA D14 | 2.77 ± 0.63 | 4.97 ± 1.05 | 3.29 ± 0.86 | 1.58 ± 0.44 |
| 2 | 3 | ATRA D14 | 8.12 ± 3.53 | 9.81 ± 5.22 | 9.72 ± 1.42 | 4.65 ± 1.05 |
| Summary Data5-150 | ||||||
| II. Long-term Reconstitution Analysis | Percentage of Donor Cells in | |||||
| Experiment # | Months Post-BMT | Culture Conditions | Gr-1 | CD11b | B220 | Thy-1.2 |
| 1 | 12 | Fresh precursors | 48.6 ± 3.65 | 61.9 ± 7.51 | 60.6 ± 6.06 | 52.6 ± 5.22 |
| 1 | 12 | No ATRA D7 | 35.8 ± 5.70 | 18.5 ± 7.99 | 32.6 ± 9.92 | 23.4 ± 8.59 |
| 1 | 12 | ATRA D7 | 33.2 ± 3.40 | 17.5 ± 9.26 | 30.9 ± 8.71 | 16.4 ± 6.67 |
| 1 | 12 | No ATRA D14 | 2.35 ± 0.51 | 1.42 ± 0.31 | 3.87 ± 0.38 | 0.35 ± 0.04 |
| 1 | 12 | ATRA D14 | 3.28 ± 0.88 | 4.20 ± 0.78 | 12.7 ± 2.65 | 4.58 ± 2.12 |
| 2 | 6 | Fresh precursors | 31.5 ± 5.46 | 40.7 ± 8.32 | 39.7 ± 6.71 | 28.7 ± 6.65 |
| 2 | 6 | No ATRA D7 | 20.0 ± 5.88 | 20.3 ± 7.72 | 30.2 ± 7.42 | 21.2 ± 7.24 |
| 2 | 6 | ATRA D7 | 28.5 ± 6.10 | 31.8 ± 8.85 | 40.5 ± 7.90 | 33.2 ± 8.05 |
| 2 | 6 | No ATRA D14 | 3.66 ± 1.25 | 0.98 ± 0.37 | 4.82 ± 2.25 | 0.42 ± 0.16 |
| 2 | 6 | ATRA D14 | 7.74 ± 5.09 | 7.73 ± 6.36 | 9.08 ± 3.09 | 3.66 ± 1.31 |
| . | Summary Data5-150 . | |||||
|---|---|---|---|---|---|---|
| I. Short-term Reconstitution Analysis . | Percentage of Donor Cells in . | |||||
| Experiment # . | Months Post-BMT . | Culture Conditions . | Gr-1 . | CD11b . | B220 . | Thy-1.2 . |
| 1 | 3 | Fresh precursors | 36.3 ± 3.84 | 41.5 ± 4.39 | 51.0 ± 6.44 | 32.6 ± 5.20 |
| 1 | 3 | No ATRA D7 | 23.5 ± 7.72 | 23.8 ± 8.08 | 32.1 ± 9.89 | 25.5 ± 8.32 |
| 1 | 3 | ATRA D7 | 20.4 ± 5.85 | 22.1 ± 7.63 | 33.9 ± 6.38 | 24.9 ± 5.69 |
| 1 | 3 | No ATRA D14 | ND | ND | ND | ND |
| 1 | 3 | ATRA D14 | 8.77 ± 1.03 | 6.68 ± 1.26 | 28.6 ± 4.06 | 10.5 ± 2.59 |
| 2 | 3 | Fresh precursors | 43.5 ± 3.25 | 49.9 ± 6.13 | 54.2 ± 4.43 | 43.0 ± 3.78 |
| 2 | 3 | No ATRA D7 | 19.9 ± 5.08 | 20.0 ± 8.24 | 34.3 ± 7.43 | 20.9 ± 4.26 |
| 2 | 3 | ATRA D7 | 35.5 ± 6.29 | 30.0 ± 9.66 | 51.7 ± 6.81 | 32.5 ± 5.55 |
| 2 | 3 | No ATRA D14 | 2.77 ± 0.63 | 4.97 ± 1.05 | 3.29 ± 0.86 | 1.58 ± 0.44 |
| 2 | 3 | ATRA D14 | 8.12 ± 3.53 | 9.81 ± 5.22 | 9.72 ± 1.42 | 4.65 ± 1.05 |
| Summary Data5-150 | ||||||
| II. Long-term Reconstitution Analysis | Percentage of Donor Cells in | |||||
| Experiment # | Months Post-BMT | Culture Conditions | Gr-1 | CD11b | B220 | Thy-1.2 |
| 1 | 12 | Fresh precursors | 48.6 ± 3.65 | 61.9 ± 7.51 | 60.6 ± 6.06 | 52.6 ± 5.22 |
| 1 | 12 | No ATRA D7 | 35.8 ± 5.70 | 18.5 ± 7.99 | 32.6 ± 9.92 | 23.4 ± 8.59 |
| 1 | 12 | ATRA D7 | 33.2 ± 3.40 | 17.5 ± 9.26 | 30.9 ± 8.71 | 16.4 ± 6.67 |
| 1 | 12 | No ATRA D14 | 2.35 ± 0.51 | 1.42 ± 0.31 | 3.87 ± 0.38 | 0.35 ± 0.04 |
| 1 | 12 | ATRA D14 | 3.28 ± 0.88 | 4.20 ± 0.78 | 12.7 ± 2.65 | 4.58 ± 2.12 |
| 2 | 6 | Fresh precursors | 31.5 ± 5.46 | 40.7 ± 8.32 | 39.7 ± 6.71 | 28.7 ± 6.65 |
| 2 | 6 | No ATRA D7 | 20.0 ± 5.88 | 20.3 ± 7.72 | 30.2 ± 7.42 | 21.2 ± 7.24 |
| 2 | 6 | ATRA D7 | 28.5 ± 6.10 | 31.8 ± 8.85 | 40.5 ± 7.90 | 33.2 ± 8.05 |
| 2 | 6 | No ATRA D14 | 3.66 ± 1.25 | 0.98 ± 0.37 | 4.82 ± 2.25 | 0.42 ± 0.16 |
| 2 | 6 | ATRA D14 | 7.74 ± 5.09 | 7.73 ± 6.36 | 9.08 ± 3.09 | 3.66 ± 1.31 |
Irradiated Ly5.1 recipients (10 mice per group) were transplanted with 1 × 105 normal Ly5.1 bone marrow cells together with 1000 fresh Ly5.2 lin− c-kit+ Sca-1+ cells (fresh precursors) or with all cells that grew from 1000 of these precursors after 7 (D7) or 14 (D14) days in liquid culture suspension. The culture medium was supplemented with 100 ng/mL SCF, IL-6, Flt-3L, and 10 ng/ml LIL-11, without (No ATRA) or with (ATRA) 1 μmol ATRA. Levels of reconstitution at 3, 6, and 12 months after transplant are shown as a summary of groups.
Mean ± SEM % donor derived nucleated peripheral blood cells identified by anti-Ly5.2. Background (peripheral blood cells of nontransplanted Ly5.1 female mice) staining was < 3.0%.
ND = Not done. Number of mice per group = 10, except No ATRA 2 weeks experiment #1 (n = 9) and No ATRA 2 weeks experiment #2 (n = 8).