ATRA effects on cell number of cultured hematopoietic precursors
| Days in Culture . | ATRA . | Cell Counts . | |
|---|---|---|---|
| Experiment 1 . | Experiment 2 . | ||
| 7 | − | 0.77 × 106 | 1.18 × 106 |
| 7 | + | 0.45 × 106 | 0.37 × 106 |
| 14 | − | 2.88 × 106 | 1.96 × 106 |
| 14 | + | 2.58 × 106 | 1.42 × 106 |
| Days in Culture . | ATRA . | Cell Counts . | |
|---|---|---|---|
| Experiment 1 . | Experiment 2 . | ||
| 7 | − | 0.77 × 106 | 1.18 × 106 |
| 7 | + | 0.45 × 106 | 0.37 × 106 |
| 14 | − | 2.88 × 106 | 1.96 × 106 |
| 14 | + | 2.58 × 106 | 1.42 × 106 |
The indicated cell counts represent all cells that grew in culture from 1000 lin− c-kit+ Sca-1+ cells after 7 or 14 days in liquid suspension culture. These cells were injected into each lethally irradiated mouse in the competitive repopulation transplant. The culture medium was supplemented with 100 ng/mL SCF, IL-6, Flt-3L, and 10 ng/mL IL-11, without (−) or with (+) 1 μmol ATRA.