The inhibition of the effect of SDF-1 by T22 in serum free cultures
| . | No. MK Colonies (per 105 cells) . | ||
|---|---|---|---|
| TPO . | TPO + SDF-1 . | TPO + T22 . | |
| whole marrow cells | 18 ± 3 | 34 ± 76-150 | 21 ± 3NS |
| Density gradient | 42 ± 6 | 74 ± 126-150 | 50 ± 2NS |
| FACS sorting | |||
| exp. 1 | 500 ± 25 | 800 ± 1006-150 | 525 ± 100NS |
| exp. 2 | 400 ± 100 | 900 ± 756-150 | 425 ± 100NS |
| . | No. MK Colonies (per 105 cells) . | ||
|---|---|---|---|
| TPO . | TPO + SDF-1 . | TPO + T22 . | |
| whole marrow cells | 18 ± 3 | 34 ± 76-150 | 21 ± 3NS |
| Density gradient | 42 ± 6 | 74 ± 126-150 | 50 ± 2NS |
| FACS sorting | |||
| exp. 1 | 500 ± 25 | 800 ± 1006-150 | 525 ± 100NS |
| exp. 2 | 400 ± 100 | 900 ± 756-150 | 425 ± 100NS |
Cells in each purification step were plated in serum-depleted medium with TPO and SDF-1 with or without T22. The results represent the number of CFU-MK-derived colonies (mean ± SEM of triplicate plates).
P < 0.05 compared to TPO alone; NS not significant compared to cultures containing TPO alone.