Sequence analysis of clonal Ig gene rearrangements obtained from three cases of B-CLL with HRS-like cells
| Pt. . | Cell Type . | VH . | VL . | HRS-Like and CLL Cells: Clonally Related? . | ||||
|---|---|---|---|---|---|---|---|---|
| VH Gene . | Mut.(%) . | Potentially Functional . | VL Gene . | Mut.(%) . | Potentially Functional . | |||
| 1 | HRS-like | DP63 (4) | 6.6 | + | 3r (Vλ3) | 5.3 | + | Yes |
| 3a (Vλ3) | 13.3 | − | ||||||
| B-CLL | DP63 (4) | 7.3 | + | 3r (Vλ3) | 6.7 | + | ||
| 3a (Vλ3) | 15.3 | − | ||||||
| 2 | HRS-like | V3-30 (3) | 8.2 | +4-150 | GLL150 (Vλ3) | 9.5 | − | No |
| B-CLL | hv3005 (3) | 0 | + | lv318 (Vλ3) | 0 | + | ||
| O1/O11 (Vκ2) | 0 | − | ||||||
| L2 (Vκ3) | 0 | − | ||||||
| 3 | HRS-like | VH4.16 (4) | 9.1 | + | 1c-1 (Vλ1) | 5.0 | − | No |
| B-CLL | V3-23 (3) | 0 | + | 1c-2 (Vλ1) | 0 | + | ||
| DP-48 (3) | 0.5 | − | ||||||
| Pt. . | Cell Type . | VH . | VL . | HRS-Like and CLL Cells: Clonally Related? . | ||||
|---|---|---|---|---|---|---|---|---|
| VH Gene . | Mut.(%) . | Potentially Functional . | VL Gene . | Mut.(%) . | Potentially Functional . | |||
| 1 | HRS-like | DP63 (4) | 6.6 | + | 3r (Vλ3) | 5.3 | + | Yes |
| 3a (Vλ3) | 13.3 | − | ||||||
| B-CLL | DP63 (4) | 7.3 | + | 3r (Vλ3) | 6.7 | + | ||
| 3a (Vλ3) | 15.3 | − | ||||||
| 2 | HRS-like | V3-30 (3) | 8.2 | +4-150 | GLL150 (Vλ3) | 9.5 | − | No |
| B-CLL | hv3005 (3) | 0 | + | lv318 (Vλ3) | 0 | + | ||
| O1/O11 (Vκ2) | 0 | − | ||||||
| L2 (Vκ3) | 0 | − | ||||||
| 3 | HRS-like | VH4.16 (4) | 9.1 | + | 1c-1 (Vλ1) | 5.0 | − | No |
| B-CLL | V3-23 (3) | 0 | + | 1c-2 (Vλ1) | 0 | + | ||
| DP-48 (3) | 0.5 | − | ||||||
Clonal V gene sequences from the HRS-like and B-CLL cells are compared to the most homologous germline genes.35,36,39 40 The V gene families are given in parentheses after the gene name. Sequences are available from the EMBL/GenBank databases under accession number AJ242555-AJ242572.
The − sign indicates a nonfunctional rearrangement, and the + indicates a potentially functional rearrangement. Clonally unrelated rearrangements of the Vλ1 gene1c are represented by 1c-1 and 1c-2, which were amplified from HRS-like and B-CLL cells, respectively.
Indicates potentially functional rearrangement rendered nonfunctional due to a stop codon and a 1-bp deletion introduced by somatic mutation (Figure 3).