Immunophenotype of HRS-like cells and B cells of the CLL
| Patient . | Cell Type . | CD30 . | CD15 . | CD20 . | CD3 . | CD5 . | Igλ* . | EBV† . |
|---|---|---|---|---|---|---|---|---|
| 1 | HRS-like | + | + | − | − | − | − | − |
| B-CLL | − | − | + | − | + | + | − | |
| 2 | HRS-like | + | − | − | − | −/+ | − | + |
| B-CLL | − | − | + | − | + | + | − | |
| 3 | HRS-like | + | + | − | − | − | − | + |
| B-CLL | − | − | + | − | + | + | − |
| Patient . | Cell Type . | CD30 . | CD15 . | CD20 . | CD3 . | CD5 . | Igλ* . | EBV† . |
|---|---|---|---|---|---|---|---|---|
| 1 | HRS-like | + | + | − | − | − | − | − |
| B-CLL | − | − | + | − | + | + | − | |
| 2 | HRS-like | + | − | − | − | −/+ | − | + |
| B-CLL | − | − | + | − | + | + | − | |
| 3 | HRS-like | + | + | − | − | − | − | + |
| B-CLL | − | − | + | − | + | + | − |
HRS-like cells were either negative or stained for both κ and λ, indicating unspecific adsorption of serum Ig. B-CLL cells were negative for κ light chain expression.
Indicates that the presence (+) or absence (−) of EBV was determined by staining with an antibody against LMP1 and EBER in situ hybridization (giving concordant results).
In patient 2, −/+ indicates that a small fraction of the cells are CD5-positive.
Very few solitary small cells were EBV-positive in the patients.