Calculations of changes in CRU numbers during culture
| Exp.No. . | Duration of Culture in Days . | CRU Data From 3-Month Posttransplant Analyses . | CRU Data From 6-Month Posttransplant Analyses . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Input . | Yield . | FoldChange . | Undivided Fraction No. (% of Total) . | Divided Fraction No. (% of Total) . | Input . | Yield . | FoldChange . | Undivided Fraction No. (% of Total) . | Divided Fraction No. (% of Total) . | ||
| 1 | 3 | 24 | 4 | 0.2 | 4 (95) | 0.2 (5) | 12 | 4 | 0.3 | 4 (95) | 0.2 (5) |
| 6 | 3 | 460 | 53 | 0.1 | 46 (87) | 7 (13) | 230 | 53 | 0.2 | 46 (87) | 7 (13) |
| mean | 0.2 | (91) | (9) | mean | 0.3 | (91) | (9) | ||||
| 2 | 4 | 3 | 12 | 4 | <0.5 (<4) | 12 (>96) | 3 | 16 | 5 | <0.5 (<3) | 16 (>97) |
| 5 | 4 | 10 | 44 | 4 | <2 (<5) | 42 (>95) | 8 | 56 | 7 | <2 (<4) | 54 (>96) |
| 7 | 4 | 10 | 11 | 1 | <1 (<9) | 10 (>91) | 8 | 14 | 2 | <1 (<7) | 13 (>93) |
| mean ± SEM | 3 ± 1 | (<6) | (>94) | mean ± SEM | 5 ± 2 | (<5) | (>95) | ||||
| 3 | 10 | 84 | 22 | 0.3 | <0.4 (<2) | 22 (>98) | 90 | 18 | 0.2 | <0.4 (<2) | 18 (>98) |
| 4 | 10 | 67 | 18 | 0.3 | <0.6 (<3) | 17 (>97) | 72 | 15 | 0.2 | <0.6 (<4) | 14 (>96) |
| mean | 0.3 | (<3) | (>97) | mean | 0.2 | (<3) | (>97) | ||||
| Exp.No. . | Duration of Culture in Days . | CRU Data From 3-Month Posttransplant Analyses . | CRU Data From 6-Month Posttransplant Analyses . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Input . | Yield . | FoldChange . | Undivided Fraction No. (% of Total) . | Divided Fraction No. (% of Total) . | Input . | Yield . | FoldChange . | Undivided Fraction No. (% of Total) . | Divided Fraction No. (% of Total) . | ||
| 1 | 3 | 24 | 4 | 0.2 | 4 (95) | 0.2 (5) | 12 | 4 | 0.3 | 4 (95) | 0.2 (5) |
| 6 | 3 | 460 | 53 | 0.1 | 46 (87) | 7 (13) | 230 | 53 | 0.2 | 46 (87) | 7 (13) |
| mean | 0.2 | (91) | (9) | mean | 0.3 | (91) | (9) | ||||
| 2 | 4 | 3 | 12 | 4 | <0.5 (<4) | 12 (>96) | 3 | 16 | 5 | <0.5 (<3) | 16 (>97) |
| 5 | 4 | 10 | 44 | 4 | <2 (<5) | 42 (>95) | 8 | 56 | 7 | <2 (<4) | 54 (>96) |
| 7 | 4 | 10 | 11 | 1 | <1 (<9) | 10 (>91) | 8 | 14 | 2 | <1 (<7) | 13 (>93) |
| mean ± SEM | 3 ± 1 | (<6) | (>94) | mean ± SEM | 5 ± 2 | (<5) | (>95) | ||||
| 3 | 10 | 84 | 22 | 0.3 | <0.4 (<2) | 22 (>98) | 90 | 18 | 0.2 | <0.4 (<2) | 18 (>98) |
| 4 | 10 | 67 | 18 | 0.3 | <0.6 (<3) | 17 (>97) | 72 | 15 | 0.2 | <0.6 (<4) | 14 (>96) |
| mean | 0.3 | (<3) | (>97) | mean | 0.2 | (<3) | (>97) | ||||
The yield is calculated as the sum of the number of CRU detected separately in the divided and undivided fractions. The numbers in parentheses indicate the number of CRU present in the fraction indicated, expressed as a percent of the total number of cells recovered from the cultures.