Each line represents one injected mouse. The data shown here include the mice described in Figure 7. Mice were injected with 2 to 5 × 10⁶ unfractionated mononuclear cells from peripheral blood of myeloma patients with aggressive disease (A) or from unfractionated mononuclear cells from G-CSF mobilized blood (B). The harvested femora yielded 4 to 5 × 10⁶ BMC, and the spleens yielded about 10⁷ cells, of which the indicated % were clonotypic.

For patient 9, of 13 mice injected, 7 had detectable clonotypic transcripts in purified RNA, 2 of 3 at 4 weeks, 2 of 3 at 8 weeks, and 3 of 7 at 12 weeks. At 12 weeks, 2 mice of the mice lacking detectable transcripts had very brittle bones. Four of the mice with detectable clonotypic transcripts in RNA also had transcripts detectable at limiting dilution, 2 of 4 in BM, and all 4 in spleen.

The values indicate the number of weeks between injection and sacrifice. Mice from patient 9 were intentionally killed at the indicated time point. Mice from other patients were killed when they became symptomatic.