Effect of IL-15 pretreatment on induction of apoptosis by CH11 mAb in myeloma cell lines
| Cell Line . | Percentages of Apoptotic Cells . | |||
|---|---|---|---|---|
| Without IL-15 Pretreatment CH11 mAb . | With IL-15 Pretreatment CH11 mAb . | |||
| − . | + . | − . | + . | |
| ARH-77 | 13 ± 1.4 | 28 ± 1.3 | 12 ± 1.8 | 15 ± 1.9 |
| IM-9 | 16 ± 1.0 | 36 ± 2.7 | 13 ± 1.3 | 20 ± 2.1 |
| LP-1 | 14 ± 1.8 | 30 ± 2.5 | 12 ± 2.6 | 19 ± 1.2 |
| MC-Car | 19 ± 1.6 | 32 ± 1.8 | 16 ± 1.4 | 23 ± 2.8 |
| OPM-2 | 18 ± 1.3 | 32 ± 1.3 | 16 ± 1.6 | 25 ± 1.4 |
| RPMI 8226 | 14 ± 1.0 | 36 ± 2.9 | 12 ± 1.3 | 18 ± 2.0 |
| Cell Line . | Percentages of Apoptotic Cells . | |||
|---|---|---|---|---|
| Without IL-15 Pretreatment CH11 mAb . | With IL-15 Pretreatment CH11 mAb . | |||
| − . | + . | − . | + . | |
| ARH-77 | 13 ± 1.4 | 28 ± 1.3 | 12 ± 1.8 | 15 ± 1.9 |
| IM-9 | 16 ± 1.0 | 36 ± 2.7 | 13 ± 1.3 | 20 ± 2.1 |
| LP-1 | 14 ± 1.8 | 30 ± 2.5 | 12 ± 2.6 | 19 ± 1.2 |
| MC-Car | 19 ± 1.6 | 32 ± 1.8 | 16 ± 1.4 | 23 ± 2.8 |
| OPM-2 | 18 ± 1.3 | 32 ± 1.3 | 16 ± 1.6 | 25 ± 1.4 |
| RPMI 8226 | 14 ± 1.0 | 36 ± 2.9 | 12 ± 1.3 | 18 ± 2.0 |
Cells were incubated with or without IL-15 (10 ng/mL) for 16 hours prior to treatment with CH11 mAb (50 ng/mL) for 24 hours. The percentages of apoptotic cells were determined using the annexin V/FITC/PI assay. Mean values of four independent experiments ± SEM are given. Induction of apoptosis by CH11 mAb treatment is significantly different in myeloma cells without or with IL-15 pretreatment as determined by the Fisher PLSD test (ARH-77:P = .038; IM-9: P = .035; LP-1: P = .018; MC-Car: P = .01; OPM-2: P = .007; RPMI 8226:P = .004).