Percentage of CD34+ cells and CFC in S phase following coculture with FN in the presence or absence of supraphysiological concentrations of cytokines
| Condition . | FN (CFC) . | FN (CD34+) . | PLL (CFC) . | PLL (CD34+) . |
|---|---|---|---|---|
| None | 3 ± 2* | 11.5 ± 2* | 35 ± 3 | 23 ± 3.4 |
| IL3 | 28 ± 5 | 25 ± 2.8 | 30 ± 5 | 26.4 ± 4 |
| GM-CSF | 27 ± 6 | — | 28 ± 6 | — |
| SCF | 31 ± 6 | 23 ± 3.5 | 34 ± 6 | 23 ± 2.9 |
| Flt3-L | 32 ± 7 | — | 29 ± 7 | — |
| Condition . | FN (CFC) . | FN (CD34+) . | PLL (CFC) . | PLL (CD34+) . |
|---|---|---|---|---|
| None | 3 ± 2* | 11.5 ± 2* | 35 ± 3 | 23 ± 3.4 |
| IL3 | 28 ± 5 | 25 ± 2.8 | 30 ± 5 | 26.4 ± 4 |
| GM-CSF | 27 ± 6 | — | 28 ± 6 | — |
| SCF | 31 ± 6 | 23 ± 3.5 | 34 ± 6 | 23 ± 2.9 |
| Flt3-L | 32 ± 7 | — | 29 ± 7 | — |
Data are shown as mean ± SEM. CFC indicates colony-forming cells; FN, fibronectin; PLL, poly-L-lysine; IL3, interleukin-3; GM-CSF, granulocyte-macrophage–colony stimulating factor; SCF, stem cell factor; and Flt3-L, fetal liver tyrosine kinase-3 ligand.
P < .01.