Regulation of surface molecules by DSP30 and CD40 ligation in normal B cells
| . | DSP30 . | CD40LF-cells . | P Value (1) . | CD40LF-cells/DSP30 . | P Value (2) . | |||
|---|---|---|---|---|---|---|---|---|
| Mean MFIR Induction . | SEM +/− . | Mean MFIR Induction . | SEM +/− . | Mean MFIR Induction . | SEM +/− . | |||
| CD40 | 2.3 | 0.2 | 1.4 | 0.2 | .13 | 1.9 | 0.2 | .38 |
| CD80 | 2.3 | 0.4 | 3.0 | 0.3 | .39 | 3.9 | 0.4 | .06 |
| CD86 | 4.6 | 0.7 | 6.7 | 1.7 | .41 | 10.7 | 3.0 | .09 |
| MHC class I | 1.7 | 0.2 | 1.5 | 0.3 | .59 | 2.3 | 0.8 | .94 |
| CD58 | 3.3 | 0.5 | 4.5 | 0.8 | .75 | 6.3 | 0.8 | .06 |
| CD54 | 2.0 | 0.2 | 13.3 | 2.3 | .03 | 12.6 | 3.2 | .31 |
| CD95 | 0.9 | 0.1 | 6.4 | 1.2 | .03 | 1.4 | 0.2 | .03 |
| . | DSP30 . | CD40LF-cells . | P Value (1) . | CD40LF-cells/DSP30 . | P Value (2) . | |||
|---|---|---|---|---|---|---|---|---|
| Mean MFIR Induction . | SEM +/− . | Mean MFIR Induction . | SEM +/− . | Mean MFIR Induction . | SEM +/− . | |||
| CD40 | 2.3 | 0.2 | 1.4 | 0.2 | .13 | 1.9 | 0.2 | .38 |
| CD80 | 2.3 | 0.4 | 3.0 | 0.3 | .39 | 3.9 | 0.4 | .06 |
| CD86 | 4.6 | 0.7 | 6.7 | 1.7 | .41 | 10.7 | 3.0 | .09 |
| MHC class I | 1.7 | 0.2 | 1.5 | 0.3 | .59 | 2.3 | 0.8 | .94 |
| CD58 | 3.3 | 0.5 | 4.5 | 0.8 | .75 | 6.3 | 0.8 | .06 |
| CD54 | 2.0 | 0.2 | 13.3 | 2.3 | .03 | 12.6 | 3.2 | .31 |
| CD95 | 0.9 | 0.1 | 6.4 | 1.2 | .03 | 1.4 | 0.2 | .03 |
Cells were cultured for 48 hours. MFIR and MFIR-induction were calculated as described in “Material and Methods” section. MFIR induction is presented as mean ± SEM of 11 samples from B-CLL patients (Table 1A) and 7 normal control subjects (Table 1B). The Wilcoxon signed rank test was used to determine statistically significant differences, comparing the MFIR induced by DSP30 stimulation versus CD40-ligation (P value (1)). A secondP value (P value (2)) was determined by testing for a difference between the combination of both stimuli and the maximum MFIR-induction of either stimulus alone. The Bonferroni-Holm correction was used to adjust significance levels. Statistical significantP values are indicated by asterisks. CLL = chronic lymphocytic leukemia; MFIR = mean fluorescence intensity ratio.