Effect of c-kit/SCF interactions on the expansion of NK cell precursors
| Genotype . | Total . | IL-2Rβ+ NK1.1− . | IL-2Rβ+ NK1.1+ . | Specific Lysis . |
|---|---|---|---|---|
| wt | 43.7 | 40.2 | 35.0 | 68% |
| W/W | 13.4 | 11.6 | 10.9 | 73% |
| Genotype . | Total . | IL-2Rβ+ NK1.1− . | IL-2Rβ+ NK1.1+ . | Specific Lysis . |
|---|---|---|---|---|
| wt | 43.7 | 40.2 | 35.0 | 68% |
| W/W | 13.4 | 11.6 | 10.9 | 73% |
Day 13 FL cells were plated at 1000 cells/well and cultured for 8 days in SCF (100 ng/mL), IL-7 (50 ng/mL), and flk2/flt3L (100 ng/mL). Thereafter, cytokines were removed, and cells were cultured for an additional 8 days in IL-2 (1000 units/mL). At the end of the culture period, cells were enumerated, stained with mAbs specific for IL-2Rβ and NK1.1, and tested in cytotoxic assays against YAC targets at an E:T ratio of 9:1. Cell numbers are expressed in ×103/well. Results are representative of two independent experiments.