Cell No (×105) in primary lymphoid organs of RAG2/γc chimeras reconstituted with W/W or wt FL cells
| Genotype of Fetal Liver . | Thymus Cells . | Bone Marrow Cells . | |||||
|---|---|---|---|---|---|---|---|
| Total . | Lymphocytes . | Total . | Lymphocytes . | NK1+CD3− . | Pre-B . | Mature B . | |
| Nonreconstituted (n = 4) | 0.4 ± 0.1 | ND | 72 ± 3 | 3.6 ± 1.7 | ND | ND | ND |
| wt (n = 7) | 452 ± 94 | 398 ± 94 | 106 ± 15 | 26 ± 5 | 0.31 ± 0.15 | 6.12 ± 2.2 | 11.9 ± 1.6 |
| W/W (n = 7) | 39 ± 24* | 32 ± 23* | 108 ± 8 | 23 ± 5 | 0.13 ± 0.04* | 4.72 ± 1.0 | 8.4 ± 1.9 |
| Genotype of Fetal Liver . | Thymus Cells . | Bone Marrow Cells . | |||||
|---|---|---|---|---|---|---|---|
| Total . | Lymphocytes . | Total . | Lymphocytes . | NK1+CD3− . | Pre-B . | Mature B . | |
| Nonreconstituted (n = 4) | 0.4 ± 0.1 | ND | 72 ± 3 | 3.6 ± 1.7 | ND | ND | ND |
| wt (n = 7) | 452 ± 94 | 398 ± 94 | 106 ± 15 | 26 ± 5 | 0.31 ± 0.15 | 6.12 ± 2.2 | 11.9 ± 1.6 |
| W/W (n = 7) | 39 ± 24* | 32 ± 23* | 108 ± 8 | 23 ± 5 | 0.13 ± 0.04* | 4.72 ± 1.0 | 8.4 ± 1.9 |
Eight to ten weeks posttransfer, thymus and BM (one femur) cells of transplanted mice and control nonreconstituted RAG2/γcchimeras were enumerated and stained with a combination of either α-CD8FITC and α-CD4TRIC, α-CD3FITC and α-NK1.1PE, or α-CD19FITC and α-IgMPE mAbs. Lymphoid gates were set based on forward and side scatter parameters, and the absolute numbers were calculated as follows: total cell No × percent of lymphoid cells × percent of cells stained positive for a given marker. Pre-B cells were defined as CD19+IgM−, and mature B cells were defined as CD19+ IgM+.
ND indicates not detected.
Indicates P < .05 for W/W versus wt chimeras.