Deduced protein sequences of isolated FVIII light-chain–specific scFv
| Heavy chains |
| FR1 CDR1 FR2 CDR2 FR3 CDR3 FR4 |
| ------------------------------ ----- -------------- ----------------- -------------------------------- ----------------------- ----------- |
| 111 |
| 12 3 4 56 789001 |
| 123456789012345678901234567890 12345 67890123456789 012a3456789012345 67890123456789012abc345678901234 567890abcdefghijklmno12 34567890123 |
| DP-10 QVQLVQSGAEVKKPGSSVKVSCKASGGTFS SYAIS WVRQAPGOGLEWMG GIIPIFGTANYAQKFQG RVTITADESTSTAYMELSSLRSEDTAVYYCAR |
| EL-14 ----------A---------------D--N -FP-- -------------- -------STK------- ---M---G---------N--------I----- QQNGGWYEGPLLE..PRPDALDI WGQGTMVTVSS |
| DP-14 QVQLVQSGAEVKKPGASVKVSCKASGYTFT SYGIS WVRQAPGQGLEWMG WISAYNGNTNYAQKLQG RVTMTTDTSTSTAYMELRSLRSDDTAVYYCAR |
| EL-5 ---- L--AT--------M----M----P-- --D-- ------------V- ---------H----F-- ---------RR--------------------- DGGGGAYEDVWSGEYPEYYAMDV WGQGTTVTVSS |
| EL-16 ---- L--AT--------M----M----P-- --D-- -------------- ---I-S---D----F-- ---------RR--------------------- ----------------------- ----------- |
| EL-25 ---- L--A---R---------------P-- --D-- -------------- ---I-S---D----F-- ---------RR--------------------- ----------------------- ----------- |
| DP-88 QVQLVQSGAEVKKPGSSVKVSCKASGGTFS SYAIS WVRQAPGQGLEWMG GIIPIFGTANYAQKFQG RVTITADKSTSTAYMELSSLRSEDTAVYYCAR |
| EL-9 -----------------------T----L- ----- ---------P--I- ------D-SKS--R--D ------NI----T-------------M-F-V- GASGIRYIDWP...PIPVDAFDI WGQGTMVTVSS |
| Light chains |
| FR1 CDR1 FR2 CDR2 FR3 CDR3 FR4 |
| ----------------------- ----------- --------------- ------- -------------------------------- --------- ----------- |
| 1 |
| 12 3 4 5 678 9 0 |
| 12345678901234567890123 45678901234 567890123456789 0123456 78901234567890123456789012345678 901234567 89012345678 |
| DPK5 DIQMTQSPSSVSASVGDRVTITC RASQGISSWLA WYQQKPGKAPKLLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQANSFP |
| EL-14 --V-------------------- ----------- --------------- ------- -------------------------------- -------LT FGGGTKVEIKR |
| L12a DIQMTQSPSTLSASVGDRVTITC RASQSISSWLA WYQQKPGKAPKLLIY KASSLES GVPSRFSGSGSGTEFTLTISSLQPDDFATYYC QQYNSYS |
| EL-5 E-VL----------I-------- ---EG-YH--- --------------- -----A- -A-----------D------------------ -HL---PLT FGGGTKVEIKR |
| DPK7 DIQMTQSPSSLSASVGDRVTITC RASQGISSWLA WYQQKP EKAPKSLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQYNSYP |
| EL-25 ---------F------------- ----H-N---- ------G----L--- ---R--- -------------E------------------ --L----LT FGGGTKLEIKR |
| DPK8 DIQLTQSPSFLSASVGDRVTITC RASQGISSYLA WYQQKPGKAPKLLIY AASTLQS GVPSRFSGSGSGTEFTLTISSLQPEDFATYYC QQLNSYP |
| EL-9 ETT------S------------- ---R-L-R--- --------T------ ------- ------------------V----A---G---- --YHTISRT FGPGTKLEIKR |
| EL-16 --VM---------F----I---- -------G--- --------------- ------- --------------------G-----V----- -KY--A-WT FGQGTKVEIKR |
| Heavy chains |
| FR1 CDR1 FR2 CDR2 FR3 CDR3 FR4 |
| ------------------------------ ----- -------------- ----------------- -------------------------------- ----------------------- ----------- |
| 111 |
| 12 3 4 56 789001 |
| 123456789012345678901234567890 12345 67890123456789 012a3456789012345 67890123456789012abc345678901234 567890abcdefghijklmno12 34567890123 |
| DP-10 QVQLVQSGAEVKKPGSSVKVSCKASGGTFS SYAIS WVRQAPGOGLEWMG GIIPIFGTANYAQKFQG RVTITADESTSTAYMELSSLRSEDTAVYYCAR |
| EL-14 ----------A---------------D--N -FP-- -------------- -------STK------- ---M---G---------N--------I----- QQNGGWYEGPLLE..PRPDALDI WGQGTMVTVSS |
| DP-14 QVQLVQSGAEVKKPGASVKVSCKASGYTFT SYGIS WVRQAPGQGLEWMG WISAYNGNTNYAQKLQG RVTMTTDTSTSTAYMELRSLRSDDTAVYYCAR |
| EL-5 ---- L--AT--------M----M----P-- --D-- ------------V- ---------H----F-- ---------RR--------------------- DGGGGAYEDVWSGEYPEYYAMDV WGQGTTVTVSS |
| EL-16 ---- L--AT--------M----M----P-- --D-- -------------- ---I-S---D----F-- ---------RR--------------------- ----------------------- ----------- |
| EL-25 ---- L--A---R---------------P-- --D-- -------------- ---I-S---D----F-- ---------RR--------------------- ----------------------- ----------- |
| DP-88 QVQLVQSGAEVKKPGSSVKVSCKASGGTFS SYAIS WVRQAPGQGLEWMG GIIPIFGTANYAQKFQG RVTITADKSTSTAYMELSSLRSEDTAVYYCAR |
| EL-9 -----------------------T----L- ----- ---------P--I- ------D-SKS--R--D ------NI----T-------------M-F-V- GASGIRYIDWP...PIPVDAFDI WGQGTMVTVSS |
| Light chains |
| FR1 CDR1 FR2 CDR2 FR3 CDR3 FR4 |
| ----------------------- ----------- --------------- ------- -------------------------------- --------- ----------- |
| 1 |
| 12 3 4 5 678 9 0 |
| 12345678901234567890123 45678901234 567890123456789 0123456 78901234567890123456789012345678 901234567 89012345678 |
| DPK5 DIQMTQSPSSVSASVGDRVTITC RASQGISSWLA WYQQKPGKAPKLLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQANSFP |
| EL-14 --V-------------------- ----------- --------------- ------- -------------------------------- -------LT FGGGTKVEIKR |
| L12a DIQMTQSPSTLSASVGDRVTITC RASQSISSWLA WYQQKPGKAPKLLIY KASSLES GVPSRFSGSGSGTEFTLTISSLQPDDFATYYC QQYNSYS |
| EL-5 E-VL----------I-------- ---EG-YH--- --------------- -----A- -A-----------D------------------ -HL---PLT FGGGTKVEIKR |
| DPK7 DIQMTQSPSSLSASVGDRVTITC RASQGISSWLA WYQQKP EKAPKSLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQYNSYP |
| EL-25 ---------F------------- ----H-N---- ------G----L--- ---R--- -------------E------------------ --L----LT FGGGTKLEIKR |
| DPK8 DIQLTQSPSFLSASVGDRVTITC RASQGISSYLA WYQQKPGKAPKLLIY AASTLQS GVPSRFSGSGSGTEFTLTISSLQPEDFATYYC QQLNSYP |
| EL-9 ETT------S------------- ---R-L-R--- --------T------ ------- ------------------V----A---G---- --YHTISRT FGPGTKLEIKR |
| EL-16 --VM---------F----I---- -------G--- --------------- ------- --------------------G-----V----- -KY--A-WT FGQGTKVEIKR |
FR, framework region; CDR, complementarity-determining region. Dashes indicate sequence identity to germline. Residues encoded by JH gene segments are underlined. Sequence numbering is according to Kabat.24 Sequences are available from GenBank under accession numbers AF146400 (VH EL-5); AF146401 (VH EL-9); AF146402 (VH EL-14); AF146403 (VH EL-16); AF146404 (VH EL-25); AF146405 (VL EL-5);AF146406 (VL EL-9); AF146407 (VL EL-14); AF146408 (VL EL-16); AF146409(VL EL-25).