Gene transfer efficiency into cell subsets and clonogenic progenitors
| Component . | Gene Transfer* . | ||
|---|---|---|---|
| Day 2 . | Day 4 . | Day 6 . | |
| Total cells | 2.4% (n = 2) | 20.1% (n = 13) | 26.5% (n = 1) |
| CD34+cells | 5.1% (n = 2) | 22.4% (n = 11) | 25.4% (n = 1) |
| CD34+CD38− cells | 7.7% (n = 2) | 20.8% (n = 11) | 26.6% (n = 1) |
| CFC | 17.7% (n = 2) | 26.3% (n = 12) | 23.7% (n = 1) |
| CAFC | 7.5% (n = 2) | 5.5% (n = 2) | ND |
| Component . | Gene Transfer* . | ||
|---|---|---|---|
| Day 2 . | Day 4 . | Day 6 . | |
| Total cells | 2.4% (n = 2) | 20.1% (n = 13) | 26.5% (n = 1) |
| CD34+cells | 5.1% (n = 2) | 22.4% (n = 11) | 25.4% (n = 1) |
| CD34+CD38− cells | 7.7% (n = 2) | 20.8% (n = 11) | 26.6% (n = 1) |
| CFC | 17.7% (n = 2) | 26.3% (n = 12) | 23.7% (n = 1) |
| CAFC | 7.5% (n = 2) | 5.5% (n = 2) | ND |
Gene transfer into CFC is expressed as the ratio of G418-resistant colonies to unselected colonies; for other components, gene transfer efficiency is the percentage of cells, colonies (CFC), or hematopoietic areas (CAFC/LTC-IC) exhibiting EGFP fluorescence.
ND indicates not done.