Hematologic analysis of double mutant mice
| . | Genotype . | |||||||
|---|---|---|---|---|---|---|---|---|
| Wild Type . | LIF−/− . | IL-6−/− . | IL-11Rα−/− . | mpl−/− . | mpl−/− LIF−/− . | mpl−/− IL-6−/− . | mpl−/− IL-11Rα−/− . | |
| Bone marrow | ||||||||
| Blasts (%) | 4 ± 1 | 3 ± 0 | 4 ± 1 | 4 ± 1 | 3 ± 1 | 3 ± 1 | 3 ± 2 | 1 ± 1 |
| Promyelocytes/myelocytes (%) | 6 ± 2 | 7 ± 4 | 6 ± 3 | 7 ± 2 | 2 ± 2 | 7 ± 4 | 4 ± | 6 ± 1 |
| Metamyelocytes/neutrophils (%) | 23 ± 2 | 21 ± 7 | 25 ± 3 | 24 ± 5 | 26 ± 4 | 22 ± 9 | 28 ± 6 | 50 ± 18* |
| Lymphocytes (%) | 26 ± 3 | 24 ± 5 | 28 ± 4 | 26 ± 7 | 37 ± 5 | 28 ± 3* | 31 ± 6 | 21 ± 12* |
| Monocytes (%) | 10 ± 5 | 7 ± 1 | 7 ± 2 | 8 ± 2 | 5 ± 3 | 10 ± 4 | 8 ± 4 | 4 ± 1 |
| Eosinophils (%) | 4 ± 3 | 5 ± 4 | 4 ± 1 | 4 ± 3 | 1 ± 1 | 1 ± 1 | 1 ± 1 | 1 ± 1 |
| Nucleated erythroid cells (%) | 29 ± 6 | 33 ± 10 | 26 ± 3 | 27 ± 6 | 26 ± 4 | 29 ± 8 | 25 ± 1 | 17 ± 7* |
| Spleen | ||||||||
| Weight (mg) | 77 ± 14 | 83 ± 18 | 71 ± 22 | 70 ± 16 | 82 ± 20 | 94 ± 8 | 80 ± 12 | 75 ± 11 |
| Blasts (%) | 4 ± 2 | 2 ± 2 | 3 ± 2 | 3 ± 1 | 2 ± 1 | 2 ± 1 | 2 ± 2 | 0 |
| Promyelocytes/myelocytes (%) | 0 | 0 | 0 | 0 | 0 | 1 ± 1 | 0 | 1 ± 1 |
| Metamyelocytes/neutrophils (%) | 6 ± 3 | 3 ± 2 | 4 ± 3 | 1 ± 1 | 2 ± 1 | 2 ± 2 | 7 ± 4 | 9 ± 9 |
| Lymphocytes (%) | 82 ± 7 | 89 ± 2 | 81 ± 5 | 89 ± 6 | 83 ± 14 | 86 ± 6 | 83 ± 4 | 77 ± 10 |
| Monocytes (%) | 3 ± 3 | 3 ± 1 | 3 ± 1 | 2 ± 2 | 1 ± 1 | 4 ± 3 | 3 ± 2 | 3 ± 2 |
| Eosinophils (%) | 1 ± 1 | 1 ± 1 | 1 ± 1 | 1 ± 1 | 0 | 0 | 1 ± 1 | 0 |
| Nucleated erythroid cells (%) | 4 ± 3 | 2 ± 2 | 8 ± 5 | 4 ± 6 | 12 ± 14 | 5 ± 5 | 4 ± 3 | 10 ± 4 |
| . | Genotype . | |||||||
|---|---|---|---|---|---|---|---|---|
| Wild Type . | LIF−/− . | IL-6−/− . | IL-11Rα−/− . | mpl−/− . | mpl−/− LIF−/− . | mpl−/− IL-6−/− . | mpl−/− IL-11Rα−/− . | |
| Bone marrow | ||||||||
| Blasts (%) | 4 ± 1 | 3 ± 0 | 4 ± 1 | 4 ± 1 | 3 ± 1 | 3 ± 1 | 3 ± 2 | 1 ± 1 |
| Promyelocytes/myelocytes (%) | 6 ± 2 | 7 ± 4 | 6 ± 3 | 7 ± 2 | 2 ± 2 | 7 ± 4 | 4 ± | 6 ± 1 |
| Metamyelocytes/neutrophils (%) | 23 ± 2 | 21 ± 7 | 25 ± 3 | 24 ± 5 | 26 ± 4 | 22 ± 9 | 28 ± 6 | 50 ± 18* |
| Lymphocytes (%) | 26 ± 3 | 24 ± 5 | 28 ± 4 | 26 ± 7 | 37 ± 5 | 28 ± 3* | 31 ± 6 | 21 ± 12* |
| Monocytes (%) | 10 ± 5 | 7 ± 1 | 7 ± 2 | 8 ± 2 | 5 ± 3 | 10 ± 4 | 8 ± 4 | 4 ± 1 |
| Eosinophils (%) | 4 ± 3 | 5 ± 4 | 4 ± 1 | 4 ± 3 | 1 ± 1 | 1 ± 1 | 1 ± 1 | 1 ± 1 |
| Nucleated erythroid cells (%) | 29 ± 6 | 33 ± 10 | 26 ± 3 | 27 ± 6 | 26 ± 4 | 29 ± 8 | 25 ± 1 | 17 ± 7* |
| Spleen | ||||||||
| Weight (mg) | 77 ± 14 | 83 ± 18 | 71 ± 22 | 70 ± 16 | 82 ± 20 | 94 ± 8 | 80 ± 12 | 75 ± 11 |
| Blasts (%) | 4 ± 2 | 2 ± 2 | 3 ± 2 | 3 ± 1 | 2 ± 1 | 2 ± 1 | 2 ± 2 | 0 |
| Promyelocytes/myelocytes (%) | 0 | 0 | 0 | 0 | 0 | 1 ± 1 | 0 | 1 ± 1 |
| Metamyelocytes/neutrophils (%) | 6 ± 3 | 3 ± 2 | 4 ± 3 | 1 ± 1 | 2 ± 1 | 2 ± 2 | 7 ± 4 | 9 ± 9 |
| Lymphocytes (%) | 82 ± 7 | 89 ± 2 | 81 ± 5 | 89 ± 6 | 83 ± 14 | 86 ± 6 | 83 ± 4 | 77 ± 10 |
| Monocytes (%) | 3 ± 3 | 3 ± 1 | 3 ± 1 | 2 ± 2 | 1 ± 1 | 4 ± 3 | 3 ± 2 | 3 ± 2 |
| Eosinophils (%) | 1 ± 1 | 1 ± 1 | 1 ± 1 | 1 ± 1 | 0 | 0 | 1 ± 1 | 0 |
| Nucleated erythroid cells (%) | 4 ± 3 | 2 ± 2 | 8 ± 5 | 4 ± 6 | 12 ± 14 | 5 ± 5 | 4 ± 3 | 10 ± 4 |
Mean ± SD, n = 2 to 6 mice of each genotype.
P < .05 for comparison of data from LIF−/−, IL-6−/−, or IL-11Rα−/− mice with wild-type controls or of LIF−/− mpl−/−, IL-6−/− mpl−/−, or IL-11Rα−/− mpl−/− mice with mpl−/− data. No other statistically significant differences were observed.