Frequency of BCL-6 mutations in specific nucleotide triplets and RGYW/WRCY motifs
| Nucleotide Sequence . | Occurrence4-150 . | Number of Mutations4-151 . | Frequency of Mutation . | P‡ . |
|---|---|---|---|---|
| Triplets mutated at higher than expected frequency | ||||
| AGC | 368 | 14 | 0.038 | <.0001 |
| GCT | 2208 | 44 | 0.020 | <.0001 |
| TAC | 92 | 4 | 0.043 | <.001 |
| CTA | 368 | 10 | 0.027 | <.001 |
| GTT | 1104 | 21 | 0.019 | <.001 |
| CTG | 1472 | 26 | 0.018 | <.001 |
| TTA | 1012 | 15 | 0.015 | <.025 |
| TGC | 1472 | 21 | 0.014 | <.025 |
| Triplets mutated at lower than expected frequency | ||||
| CCC | 1564 | 3 | 0.0019 | <.01 |
| CTC | 2116 | 8 | 0.0038 | <.025 |
| GGG | 1748 | 7 | 0.0040 | <.05 |
| TCC | 1840 | 7 | 0.0038 | <.05 |
| CCT | 1656 | 6 | 0.0036 | <.05 |
| GGA | 1656 | 6 | 0.0036 | <.05 |
| GCC | 1288 | 4 | 0.0031 | <.05 |
| AAA | 1288 | 4 | 0.0031 | <.05 |
| ATG | 736 | 1 | 0.0014 | <.05 |
| AAG | 736 | 1 | 0.0014 | <.05 |
| RGYW/WRCY mutated at higher than expected frequency | ||||
| AGCT | 92 | 10 | 0.109 | <.0001 |
| AGCA | 92 | 3 | 0.033 | <.05 |
| TGCT | 552 | 20 | 0.036 | <.0001 |
| Nucleotide Sequence . | Occurrence4-150 . | Number of Mutations4-151 . | Frequency of Mutation . | P‡ . |
|---|---|---|---|---|
| Triplets mutated at higher than expected frequency | ||||
| AGC | 368 | 14 | 0.038 | <.0001 |
| GCT | 2208 | 44 | 0.020 | <.0001 |
| TAC | 92 | 4 | 0.043 | <.001 |
| CTA | 368 | 10 | 0.027 | <.001 |
| GTT | 1104 | 21 | 0.019 | <.001 |
| CTG | 1472 | 26 | 0.018 | <.001 |
| TTA | 1012 | 15 | 0.015 | <.025 |
| TGC | 1472 | 21 | 0.014 | <.025 |
| Triplets mutated at lower than expected frequency | ||||
| CCC | 1564 | 3 | 0.0019 | <.01 |
| CTC | 2116 | 8 | 0.0038 | <.025 |
| GGG | 1748 | 7 | 0.0040 | <.05 |
| TCC | 1840 | 7 | 0.0038 | <.05 |
| CCT | 1656 | 6 | 0.0036 | <.05 |
| GGA | 1656 | 6 | 0.0036 | <.05 |
| GCC | 1288 | 4 | 0.0031 | <.05 |
| AAA | 1288 | 4 | 0.0031 | <.05 |
| ATG | 736 | 1 | 0.0014 | <.05 |
| AAG | 736 | 1 | 0.0014 | <.05 |
| RGYW/WRCY mutated at higher than expected frequency | ||||
| AGCT | 92 | 10 | 0.109 | <.0001 |
| AGCA | 92 | 3 | 0.033 | <.05 |
| TGCT | 552 | 20 | 0.036 | <.0001 |
The table includes only those nucleotide triplets and RGYW/WRCY motifs for which a statistically significant P-value was observed.
Occurrence refers to the total number of times that each nucleotide triplet or RGYW/WRCY motif appeared in the total BCL-6 sequence analyzed, ie, the number of nucleotides of the BCL-6 sequence analyzed in each single case multiplied by the number of samples investigated.
Number of mutations in each individual nucleotide triplet or RGYW/WRCY motif occurring in the total BCL-6 sequence analyzed.
To determine P values, the number of mutations observed in each nucleotide triplet or RGYW/WRCY motif was compared with the probability of mutation based on random chance using the goodness-of-fit χ2 test.