Extracellular but not transmembrane activated hβc mutants induce differentiation of FDB cells.
| Cell Line . | RufPuro Construct . | Growth Factor . | Blast/Pro . | Myel/Meta . | Band/Neut . | Mono . | MK . |
|---|---|---|---|---|---|---|---|
| FDB1 | — | IL-3 | 90 | 7 | 1 | 1 | 1 |
| — | GM-CSF | 0 | 3 | 59 | 37 | 1 | |
| FIΔ | — | 1 | 5 | 51 | 44 | 0 | |
| V449E | — | 82 | 8 | 7 | 2 | 1 | |
| I374N | — | 0 | 7 | 58 | 34 | 1 | |
| FDB2 | — | IL-3 | 72 | 20 | 7 | 1 | 0 |
| — | GM-CSF | 3 | 8 | 65 | 24 | 0 | |
| FIΔ | — | 5 | 6 | 77 | 12 | 0 | |
| V449E | — | 82 | 12 | 5 | 1 | 1 | |
| I374N | — | 8 | 8 | 76 | 8 | 0 |
| Cell Line . | RufPuro Construct . | Growth Factor . | Blast/Pro . | Myel/Meta . | Band/Neut . | Mono . | MK . |
|---|---|---|---|---|---|---|---|
| FDB1 | — | IL-3 | 90 | 7 | 1 | 1 | 1 |
| — | GM-CSF | 0 | 3 | 59 | 37 | 1 | |
| FIΔ | — | 1 | 5 | 51 | 44 | 0 | |
| V449E | — | 82 | 8 | 7 | 2 | 1 | |
| I374N | — | 0 | 7 | 58 | 34 | 1 | |
| FDB2 | — | IL-3 | 72 | 20 | 7 | 1 | 0 |
| — | GM-CSF | 3 | 8 | 65 | 24 | 0 | |
| FIΔ | — | 5 | 6 | 77 | 12 | 0 | |
| V449E | — | 82 | 12 | 5 | 1 | 1 | |
| I374N | — | 8 | 8 | 76 | 8 | 0 |
Numbers are the percentage of blasts and promyelocytes (blast/pro), myelocytes and metamyelocytes (myel/meta), bands and mature neutrophils (band/neut), monocytes and macrophages (mono), and megakaryocyte-like cells (MK) of 200 cells scored at day 5 of culture.