Effect of growth factor concentration on FDB2 cells.*
| Growth Factor (U/mL) . | Blast/Pro . | Myel/Meta . | Band/Neut . | Mono . | MK . |
|---|---|---|---|---|---|
| IL-3 | |||||
| 500 | 66 | 22 | 6 | 4 | 2 |
| 50 | 63 | 23 | 9 | 4 | 1 |
| 10 | 54 | 36 | 5 | 4 | 2 |
| 2 | 39 | 47 | 8 | 6 | 2 |
| 0.4 | 0 | 28 | 43 | 29 | 0 |
| 0.08 | 0 | 30 | 51 | 19 | 0 |
| 0.016 | Nonviable† | ||||
| GM-CSF | |||||
| 500 | 4 | 17 | 68 | 12 | 0 |
| 100 | 0 | 4 | 67 | 30 | 0 |
| 20 | 0 | 0 | 63 | 37 | 0 |
| 4 | 0 | 10 | 46 | 45 | 0 |
| 0.8 | 1 | 19 | 46 | 35 | 0 |
| 0.16 | 2 | 18 | 40 | 41 | 0 |
| 0.032 | 3 | 35 | 38 | 24 | 0 |
| Growth Factor (U/mL) . | Blast/Pro . | Myel/Meta . | Band/Neut . | Mono . | MK . |
|---|---|---|---|---|---|
| IL-3 | |||||
| 500 | 66 | 22 | 6 | 4 | 2 |
| 50 | 63 | 23 | 9 | 4 | 1 |
| 10 | 54 | 36 | 5 | 4 | 2 |
| 2 | 39 | 47 | 8 | 6 | 2 |
| 0.4 | 0 | 28 | 43 | 29 | 0 |
| 0.08 | 0 | 30 | 51 | 19 | 0 |
| 0.016 | Nonviable† | ||||
| GM-CSF | |||||
| 500 | 4 | 17 | 68 | 12 | 0 |
| 100 | 0 | 4 | 67 | 30 | 0 |
| 20 | 0 | 0 | 63 | 37 | 0 |
| 4 | 0 | 10 | 46 | 45 | 0 |
| 0.8 | 1 | 19 | 46 | 35 | 0 |
| 0.16 | 2 | 18 | 40 | 41 | 0 |
| 0.032 | 3 | 35 | 38 | 24 | 0 |
Numbers are percentages of blasts and promyelocytes (blast/pro), myelocytes and metamyelocytes (myel/meta), bands and mature neutrophils (band/neut), monocytes and macrophages (mono), and megakaryocyte-like cells (MK) of 200 cells scored at day 6 of culture.
No significant viability after 6 days' culture.