Correlation between FISH and Southern blot analyses
| Percentage of Ph+ Cells . | FISH Percentage of Positive Nuclei . | Southern Blot Percentage of Rearranged Fragments . |
|---|---|---|
| 100 | 94% | 47% |
| 50 | 47% | 31% |
| 10 | 10% | 6% |
| 7 | 4% | (a) |
| 4 | 2% | (a) |
| 2 | 2% | Negative |
| 0 | Negative | Negative |
| Percentage of Ph+ Cells . | FISH Percentage of Positive Nuclei . | Southern Blot Percentage of Rearranged Fragments . |
|---|---|---|
| 100 | 94% | 47% |
| 50 | 47% | 31% |
| 10 | 10% | 6% |
| 7 | 4% | (a) |
| 4 | 2% | (a) |
| 2 | 2% | Negative |
| 0 | Negative | Negative |
Dilutions of Ph+ cells in Ph− cells were analyzed by FISH and Southern blot. Results are shown as the percentage of positive nuclei for FISH and as an estimation of the relative intensity of the rearranged bands for Southern blot; (a) represents the presence of weak bands in Southern blot that could not be quantified.