Table. Primers for single-tube multiplex-PCR analysis of common-thalassemia deletions
| Name . | 5′ → 3′ Sequence . | GenBank ID: Nucleotides . | Amplicon (Size) . |
|---|---|---|---|
| LIS1-F | GTCGTCACTGGCAGCGTAGATC | HSLIS10:407 → 428 | LIS1 3′UTR frag |
| LIS1-R | GATTCCAGGTTGTAGACGGACTG | HSLIS10:2909 → 2887 | (2503 bp) |
| α2/3.7-F | CCCCTCGCCAAGTCCACCC | HUMHBA4:5676 → 5694 | -α3.7jxn frag |
| 3.7/20.5-R | AAAGCACTCTAGGGTCCAGCG | HUMHBA4:11514 → 11494 | (2022/2029 bp) |
| α2/3.7-F | see above | α2 gene | |
| α2-R | AGACCAGGAAGGGCCGGTG | HUMHBA4:7475 → 7457 | (1800 bp) |
| 4.2-F | GGTTTACCCATGTGGTGCCTC | HUMHBA4:3064 → 3084 | -α4.2jxn frag |
| 4.2-R | CCCGTTGGATCTTCTCATTTCCC | HUMHBA4:8942 → 8920 | (1628 bp) |
| SEA-F | CGATCTGGGCTCTGTGTTCTC | HSGG1:26120 → 26140 | --SEAjxn frag |
| SEA-R | AGCCCACGTTGTGTTCATGGC | HSCOS12:3817 → 3797 | (1349 bp) |
| FIL-F | TGCAAATATGTTTCTCTCATTCTGTG | HSGG1:11684 → 11709 | --FILjxn frag |
| FIL-R | ATAACCTTTATCTGCCACATGTAGC | HSCOS12:570 → 546 | (1166 bp) |
| 20.5-F | GCCCAACATCCGGAGTACATG | HSGG1:17904 → 17924 | -(α)20.5jxn frag |
| 3.7/20.5-R | see above | (1007 bp) | |
| MED-F | TACCCTTTGCAAGCACACGTAC | HSGG1:23123 → 23144 | --MEDjxn frag |
| MED-R | TCAATCTCCGACAGCTCCGAC | HSGG1:41203 → 41183 | (807 bp) |
| Name . | 5′ → 3′ Sequence . | GenBank ID: Nucleotides . | Amplicon (Size) . |
|---|---|---|---|
| LIS1-F | GTCGTCACTGGCAGCGTAGATC | HSLIS10:407 → 428 | LIS1 3′UTR frag |
| LIS1-R | GATTCCAGGTTGTAGACGGACTG | HSLIS10:2909 → 2887 | (2503 bp) |
| α2/3.7-F | CCCCTCGCCAAGTCCACCC | HUMHBA4:5676 → 5694 | -α3.7jxn frag |
| 3.7/20.5-R | AAAGCACTCTAGGGTCCAGCG | HUMHBA4:11514 → 11494 | (2022/2029 bp) |
| α2/3.7-F | see above | α2 gene | |
| α2-R | AGACCAGGAAGGGCCGGTG | HUMHBA4:7475 → 7457 | (1800 bp) |
| 4.2-F | GGTTTACCCATGTGGTGCCTC | HUMHBA4:3064 → 3084 | -α4.2jxn frag |
| 4.2-R | CCCGTTGGATCTTCTCATTTCCC | HUMHBA4:8942 → 8920 | (1628 bp) |
| SEA-F | CGATCTGGGCTCTGTGTTCTC | HSGG1:26120 → 26140 | --SEAjxn frag |
| SEA-R | AGCCCACGTTGTGTTCATGGC | HSCOS12:3817 → 3797 | (1349 bp) |
| FIL-F | TGCAAATATGTTTCTCTCATTCTGTG | HSGG1:11684 → 11709 | --FILjxn frag |
| FIL-R | ATAACCTTTATCTGCCACATGTAGC | HSCOS12:570 → 546 | (1166 bp) |
| 20.5-F | GCCCAACATCCGGAGTACATG | HSGG1:17904 → 17924 | -(α)20.5jxn frag |
| 3.7/20.5-R | see above | (1007 bp) | |
| MED-F | TACCCTTTGCAAGCACACGTAC | HSGG1:23123 → 23144 | --MEDjxn frag |
| MED-R | TCAATCTCCGACAGCTCCGAC | HSGG1:41203 → 41183 | (807 bp) |