Adhesion of Platelets to Collagen Type I in the Absence (None) and in the Presence of Affinity-Purified IgG From Normal Human Serum, Anti-Sita, or Murine MoAb Gi9 Specific for GPIa/IIa
| Platelets Phenotypes . | IgG . | Collagen Type I (OD410) . | |
|---|---|---|---|
| 50 μg/mL . | 1 μg/mL . | ||
| Sita (−) | None | 0.79 ± 0.11 | 0.68 ± 0.11 |
| Normal serum | 1.15 ± 0.20 | 0.86 ± 0.20 | |
| Anti-Sita | 1.25 ± 0.15 | 1.01 ± 0.08 | |
| MoAb Gi9 | 0.29 ± 0.04 | 0.20 ± 0.03 | |
| Sita (+) | None | 0.85 ± 0.25 | 0.62 ± 0.18 |
| Normal serum | 1.20 ± 0.15 | 0.94 ± 0.13 | |
| Anti-Sita | 1.32 ± 0.12 | 1.01 ± 0.08 | |
| MoAb Gi9 | 0.28 ± 0.12 | 0.21 ± 0.05 | |
| Platelets Phenotypes . | IgG . | Collagen Type I (OD410) . | |
|---|---|---|---|
| 50 μg/mL . | 1 μg/mL . | ||
| Sita (−) | None | 0.79 ± 0.11 | 0.68 ± 0.11 |
| Normal serum | 1.15 ± 0.20 | 0.86 ± 0.20 | |
| Anti-Sita | 1.25 ± 0.15 | 1.01 ± 0.08 | |
| MoAb Gi9 | 0.29 ± 0.04 | 0.20 ± 0.03 | |
| Sita (+) | None | 0.85 ± 0.25 | 0.62 ± 0.18 |
| Normal serum | 1.20 ± 0.15 | 0.94 ± 0.13 | |
| Anti-Sita | 1.32 ± 0.12 | 1.01 ± 0.08 | |
| MoAb Gi9 | 0.28 ± 0.12 | 0.21 ± 0.05 | |
Biotinylated platelets from Sita (−) individuals or Sita (+) individuals (B.III.2 and 3 in Fig 2) were allowed to adhere to microtiter wells coated with either BSA (10 mg/mL) or collagen type I (50 and 1 μg/mL). Results are represented as the means of optical density (OD410) ± SD for triplicate samples. The OD410 of platelet adhesion to wells coated with BSA was less than 0.16 ± 0.07. The number of donors tested was 2.