Table 3. Adhesion of Platelets to... | American Society of Hematology

Table 3.

Adhesion of Platelets to Collagen Type I in the Absence (None) and in the Presence of Affinity-Purified IgG From Normal Human Serum, Anti-Sit^a, or Murine MoAb Gi9 Specific for GPIa/IIa

Platelets Phenotypes	IgG	Collagen Type I (OD₄₁₀)
Platelets Phenotypes	IgG	50 μg/mL	1 μg/mL
Sit^a (−)	None	0.79 ± 0.11	0.68 ± 0.11
	Normal serum	1.15 ± 0.20	0.86 ± 0.20
	Anti-Sit^a	1.25 ± 0.15	1.01 ± 0.08
	MoAb Gi9	0.29 ± 0.04	0.20 ± 0.03
Sit^a (+)	None	0.85 ± 0.25	0.62 ± 0.18
	Normal serum	1.20 ± 0.15	0.94 ± 0.13
	Anti-Sit^a	1.32 ± 0.12	1.01 ± 0.08
	MoAb Gi9	0.28 ± 0.12	0.21 ± 0.05

Platelets Phenotypes	IgG	Collagen Type I (OD₄₁₀)
Platelets Phenotypes	IgG	50 μg/mL	1 μg/mL
Sit^a (−)	None	0.79 ± 0.11	0.68 ± 0.11
	Normal serum	1.15 ± 0.20	0.86 ± 0.20
	Anti-Sit^a	1.25 ± 0.15	1.01 ± 0.08
	MoAb Gi9	0.29 ± 0.04	0.20 ± 0.03
Sit^a (+)	None	0.85 ± 0.25	0.62 ± 0.18
	Normal serum	1.20 ± 0.15	0.94 ± 0.13
	Anti-Sit^a	1.32 ± 0.12	1.01 ± 0.08
	MoAb Gi9	0.28 ± 0.12	0.21 ± 0.05

Biotinylated platelets from Sit^a (−) individuals or Sit^a (+) individuals (B.III.2 and 3 in Fig 2) were allowed to adhere to microtiter wells coated with either BSA (10 mg/mL) or collagen type I (50 and 1 μg/mL). Results are represented as the means of optical density (OD₄₁₀) ± SD for triplicate samples. The OD₄₁₀ of platelet adhesion to wells coated with BSA was less than 0.16 ± 0.07. The number of donors tested was 2.

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