Sequences of primers used for the detection of MMPs and TIMPs
| Gene* . | NT (bp) . | Sense Sequence . | Antisense Sequence . |
|---|---|---|---|
| MMP-2 | 705 | CTTGCAGGAGACAAGTTCTGG | TTAAGGTGGTGCAGGTATCTGG |
| MMP-9 | 380 | CCATGAGTCCCTGGCAG | AGTATGGATGTTATGATG |
| TIMP-1 | 655 | CTTTGCATCTCTGGCATCTGG | AAGTAGACAGTGTTCAGGC |
| TIMP-2 | 584 | TGCAGCTGCTCCCCGGTGCAC | TTATGGGTCCTCGATGTCAAG |
| TIMP-3 | 392 | AACTCCGACATCGTGATCCGGGCC | GGAGAGCATGTCGGTCCAGAGACA |
| TIMP-4 | 491 | TCGGCTCTAGTGATACGGGCC | CCTCGGTACCAGCTGCAGATG |
| Gene* . | NT (bp) . | Sense Sequence . | Antisense Sequence . |
|---|---|---|---|
| MMP-2 | 705 | CTTGCAGGAGACAAGTTCTGG | TTAAGGTGGTGCAGGTATCTGG |
| MMP-9 | 380 | CCATGAGTCCCTGGCAG | AGTATGGATGTTATGATG |
| TIMP-1 | 655 | CTTTGCATCTCTGGCATCTGG | AAGTAGACAGTGTTCAGGC |
| TIMP-2 | 584 | TGCAGCTGCTCCCCGGTGCAC | TTATGGGTCCTCGATGTCAAG |
| TIMP-3 | 392 | AACTCCGACATCGTGATCCGGGCC | GGAGAGCATGTCGGTCCAGAGACA |
| TIMP-4 | 491 | TCGGCTCTAGTGATACGGGCC | CCTCGGTACCAGCTGCAGATG |
Each pair of primers were tested for their functional integrity. For TIMP-3 and TIMP-4, the primers were tested using mRNA extracted from C6 glioma cells or freshly isolated murine spleen cells.