Fibrinogen Antigen Levels Determined by ELISA
| Cell Lines . | Cell Lysates (ng/mL) . | Media (ng/mL) . |
|---|---|---|
| γ153C (n = 12) | 1,134 ± 810 (330-3,200) | 1,995 ± 1,265 (230-4,800) |
| γ153R (n = 13) | 30 ± 31 (<10-92) | <10 (<10) |
| γ153R (n = 9*) | 41 ± 31 (<10-92) | <10 (<10) |
| γ153A (n = 6) | 107 ± 121 (<10-346) | <10 (<10) |
| Aα · Bβ (n = 3) | <10 (<10) | <10 (<10) |
| Cell Lines . | Cell Lysates (ng/mL) . | Media (ng/mL) . |
|---|---|---|
| γ153C (n = 12) | 1,134 ± 810 (330-3,200) | 1,995 ± 1,265 (230-4,800) |
| γ153R (n = 13) | 30 ± 31 (<10-92) | <10 (<10) |
| γ153R (n = 9*) | 41 ± 31 (<10-92) | <10 (<10) |
| γ153A (n = 6) | 107 ± 121 (<10-346) | <10 (<10) |
| Aα · Bβ (n = 3) | <10 (<10) | <10 (<10) |
The concentrations of fibrinogen in the cell lysates or the conditioned media from transfected CHO cells were measured by ELISA described in Materials and Methods. Data represent the mean ± SD in numbers of CHO cell lines. Numbers in parentheses mean the range of fibrinogen concentration.
Number of clones after deletion of γ-chain plasmid nonincorporated clones.