Effects of Ik7 on DC hematopoiesis
| Exp. # . | Conditions . | FKGmT4 . | FKGm17 . | ||
|---|---|---|---|---|---|
| Percentage of DC* . | Number of DC† . | Percentage of DC . | Number of DC . | ||
| 1 | Control‡ | 4.7 | 100 | 4.3 | 100 |
| Ik7 | 4.2 | 198 | 0.7 | 17 | |
| 2 | Control | 4.6 | 100 | 1.6 | 100 |
| Ik7 | 0.7 | 34 | 0.1 | 46 | |
| 3 | Control | 11 | 100 | 1.0 | 100 |
| Ik7 | 7.3 | 100 | 0.2 | 26 | |
| 4 | Control | N.T. | N.T. | 2.0 | 100 |
| Ik7 | N.T. | N.T. | 0.5 | 50 | |
| Average response1-153 | 57 ± 31 | 110 ± 67 | 16 ± 8 | 34 ± 15 | |
| Ik7 vs. control (%) ± SD | P > 0.05 | P > 0.05 | P < 0.001 | P < 0.01 | |
| Exp. # . | Conditions . | FKGmT4 . | FKGm17 . | ||
|---|---|---|---|---|---|
| Percentage of DC* . | Number of DC† . | Percentage of DC . | Number of DC . | ||
| 1 | Control‡ | 4.7 | 100 | 4.3 | 100 |
| Ik7 | 4.2 | 198 | 0.7 | 17 | |
| 2 | Control | 4.6 | 100 | 1.6 | 100 |
| Ik7 | 0.7 | 34 | 0.1 | 46 | |
| 3 | Control | 11 | 100 | 1.0 | 100 |
| Ik7 | 7.3 | 100 | 0.2 | 26 | |
| 4 | Control | N.T. | N.T. | 2.0 | 100 |
| Ik7 | N.T. | N.T. | 0.5 | 50 | |
| Average response1-153 | 57 ± 31 | 110 ± 67 | 16 ± 8 | 34 ± 15 | |
| Ik7 vs. control (%) ± SD | P > 0.05 | P > 0.05 | P < 0.001 | P < 0.01 | |
Percentages of DC are calculated by flow cytometric measurement of CD1a- or CD83-positive cells after 7 to 11 days of culture. Results are expressed as percentages of live cells excluding propidium iodide and expressing the DC markers after subtraction of the percentage of live cells stained with isotype-matched irrelevant mAb.
Numbers of DC are calculated by multiplying the percentage of DC with the total number of live cells (excluding trypan blue). Results of Ik7-infected cultures are expressed relative to control cultures that are assigned an arbitrary value of 100.
Control conditions are CD34+ EGFP+control-infected cells compared to Ik7 conditions, ie, CD34+ EGFP+ Ik7-infected cells.
Responses to Ik7 in each experiment were calculated as a percentage relative to control, which was assigned an arbitrary value of 100% and averaged ± SD.