Flow Cytometry Analysis of Surface-Marker Expression on Chemokine-Stimulated PMN Attached to Endothelial Cells
| MoAbs . | Unstimulated . | IL-8 (20 nmol/L) . | PF-4 (5 μmol/L) . |
|---|---|---|---|
| α CD11a | 17.3 ± 1.4 | 17.6 ± 3.2 | 17.5 ± 3.5 |
| α CD11b | 108.8 ± 21.0 | 208.6 ± 28.9 | 116.6 ± 22.2 |
| α CD18 | 117.7 ± 36.4 | 197.9 ± 30.5 | 123.4 ± 29.8 |
| α CD62L | 44.1 ± 17.2 | 2.5 ± 5.2 | 36.8 ± 9.4 |
| MoAbs . | Unstimulated . | IL-8 (20 nmol/L) . | PF-4 (5 μmol/L) . |
|---|---|---|---|
| α CD11a | 17.3 ± 1.4 | 17.6 ± 3.2 | 17.5 ± 3.5 |
| α CD11b | 108.8 ± 21.0 | 208.6 ± 28.9 | 116.6 ± 22.2 |
| α CD18 | 117.7 ± 36.4 | 197.9 ± 30.5 | 123.4 ± 29.8 |
| α CD62L | 44.1 ± 17.2 | 2.5 ± 5.2 | 36.8 ± 9.4 |
Neutrophils were incubated with IL-8 or PF-4 in the presence of EC as described in the legend to Fig 1. After attachment, PMN were carefully removed on ice and incubated with different MoAbs (2 μg/mL each), followed by staining with a DTAF-conjugated secondary antibody. Data are given as the MFI and represent the mean ± SD of 3 different experiments. Backgrounds (2.7 ± 0.2) obtained in the presence of an isotype control were subtracted.