The effect of coculture of CD4 and APC with Tc1 and Tc2 CD8 clones on subsequent cloning frequency, subset distribution, and average levels of cytokine production of CD4 clones
| . | Cloning Frequency . | Subset . | Geometric Mean Levels of Cytokine (pg/mL) . | |||
|---|---|---|---|---|---|---|
| Th1 (%) . | Th2 (%) . | IFN-γ . | IL-4 . | IL-5 . | ||
| Control | 0.27 | 13 | 17 | 720 | 757 | 282 |
| +Tc1 | 0.17 | 50 | 6 | 3308 | 342 | 182 |
| +Tc2 | 0.07 | 0 | 43 | 514 | 1149 | 780 |
| . | Cloning Frequency . | Subset . | Geometric Mean Levels of Cytokine (pg/mL) . | |||
|---|---|---|---|---|---|---|
| Th1 (%) . | Th2 (%) . | IFN-γ . | IL-4 . | IL-5 . | ||
| Control | 0.27 | 13 | 17 | 720 | 757 | 282 |
| +Tc1 | 0.17 | 50 | 6 | 3308 | 342 | 182 |
| +Tc2 | 0.07 | 0 | 43 | 514 | 1149 | 780 |
CD8-depleted PBMC (CD4 + APC) were stimulated with SEB for 14 days and cultured with or without Tc1 or Tc2 clones at a 1:1 ratio. At day 14, CD4 cells were purified and cloned at limiting dilution using PHA, feeders, and IL-2. After 2 rounds of mitogen stimulation, newly generated CD4 clones were screened for cytokine production and classified based on IL-4 and IFN-γ levels. Geometric mean cytokine production was calculated for clones producing a particular cytokine.