Antibodies Used for Detection of Cytokines
| Determinant . | Antibody Code . | Source . | Second Step . | Third Step . |
|---|---|---|---|---|
| IL-3 | BL-3M | Genzyme | Horse antimouse Biotin (Vector) | Streptavidin HRP |
| IL-7 | 1689-01 | Genzyme | Horse antimouse Biotin | Streptavidin HRP |
| GM-CSF | 2M-213 | Genzyme | Horse antimouse Biotin | Streptavidin HRP |
| IL-2 | 80-3418-01 | Genzyme | Horse antimouse Biotin | Streptavidin HRP |
| IL-4 | 1842-01 | Genzyme | Horse antimouse Biotin | Streptavidin HRP |
| IL-10 | B-S10 | Instruchemie | Rabbit antimouse-Ig-PO (Dako) | — |
| IL-1α | Vmp18 (biotinylated) | See legend | Streptavidin HRP (GIBCO BRL) | — |
| TNF-α | Vhp20 | See legend | Streptavidin HRP | — |
| IL-5 | 2374-u | Genzyme | Horse antimouse Biotin | Streptavidin HRP |
| INF-γ | MD-2 | See legend | Horse antimouse Biotin | Streptavidin HRP |
| Determinant . | Antibody Code . | Source . | Second Step . | Third Step . |
|---|---|---|---|---|
| IL-3 | BL-3M | Genzyme | Horse antimouse Biotin (Vector) | Streptavidin HRP |
| IL-7 | 1689-01 | Genzyme | Horse antimouse Biotin | Streptavidin HRP |
| GM-CSF | 2M-213 | Genzyme | Horse antimouse Biotin | Streptavidin HRP |
| IL-2 | 80-3418-01 | Genzyme | Horse antimouse Biotin | Streptavidin HRP |
| IL-4 | 1842-01 | Genzyme | Horse antimouse Biotin | Streptavidin HRP |
| IL-10 | B-S10 | Instruchemie | Rabbit antimouse-Ig-PO (Dako) | — |
| IL-1α | Vmp18 (biotinylated) | See legend | Streptavidin HRP (GIBCO BRL) | — |
| TNF-α | Vhp20 | See legend | Streptavidin HRP | — |
| IL-5 | 2374-u | Genzyme | Horse antimouse Biotin | Streptavidin HRP |
| INF-γ | MD-2 | See legend | Horse antimouse Biotin | Streptavidin HRP |
The monoclonal antibodies (MoAbs) for the detection of the cytokines in frozen sections, including original codes, sources, and references, are listed with corresponding second- and third-step antibodies or biotin-streptavidin reagents for validated use.8 All antibodies used are mouse MoAbs. The biotinylated MoAbs Vmp18 and Vhp20, respectively, for the detection of IL-1α and TNF-α were kindly provided by Dr P. Ghiaray (IRIS, Sienna, Italy); and the MoAb MD-2 for the detection of IFN-γ was a kind gift of Dr P. van der Meide (BPRC, Rijswijk, The Netherlands).