Five ml of fresh normal human plasma was loaded onto a 1 ml NHS-HiTrap column coupled to anti-FHRP–1-specific IgG or preimmune IgG. The first 1 ml of flow-through was collected and the concentration of FHRP and fibrinogen was measured by ELISA as described in “Methods.”

Calculated by (1 − [Imm/Preimm]) × 100, where Imm represents concentration of flow-through fraction from anti-FHRP–1 specific IgG column, and Preimm, that of preimmune IgG column.

Data are the average and the standard errors from 3 independent experiments.