Flow Cytometric Analysis of 7 Different Rh SDM Mutant K562 Lines Using Monoclonal Anti-c and E
| Anti-c/Anti-E . | K562/D . | K562/cE . | K562/lp3 . | K562/lp4 . | K562/lp3 + 6 . | K562/lp4 + 6 . | K562/lp3 + 4 . | K562/lp3 + 4 + 6 . | K562/lp6 . |
|---|---|---|---|---|---|---|---|---|---|
| Anti-c MS33 | 8.7 | 39.9 | 32.9 | 43.1 | 37.3 | 31.3 | 11.7 | 17.7 | 39.9 |
| Anti-c BS240 | 3.6 | 11.2 | 14.3 | 18.4 | 3.8 | 3.0 | 3.1 | 3.8 | 6.0 |
| Anti-c MS45 | 10.6 | 42.1 | 30.1 | 19.3 | 54.8 | 30.0 | 34.7 | 45.3 | 20.7 |
| Anti-c 951 | 8.4 | 16.1 | 5.2 | 6.8 | 7.3 | 5.3 | 3.3 | 3.7 | 10.3 |
| Anti-c MS40 | 4.4 | 10.0 | 3.7 | 3.3 | 4.9 | 4.6 | 3.5 | 3.7 | 5.3 |
| Anti-c MS51 | 9.1 | 42.1 | 27.8 | 34.7 | 30.8 | 20.7 | 10.8 | 14.1 | 24.4 |
| Anti-c MS37 | 6.4 | 30.0 | 16.0 | 32.7 | 52.0 | 24.1 | 10.6 | 11.2 | 19.2 |
| Anti-E 906 | 2.9 | 10.9 | 8.7 | 4.1 | 2.3 | 2.8 | 2.7 | 2.9 | 12.7 |
| Anti-E H98 | 3.9 | 26.0 | 2.8 | 2.8 | 2.5 | 2.9 | 2.8 | 3.0 | 2.7 |
| Anti-E H4-1 | 3.6 | 32.0 | 15.7 | 27.4 | 3.2 | 3.2 | 3.3 | 3.3 | 47.2 |
| Anti-E HIRO-18 | 3.6 | 50.1 | 22.5 | 40.8 | 3.6 | 4.3 | 3.6 | 4.2 | 41.1 |
| Anti-c/Anti-E . | K562/D . | K562/cE . | K562/lp3 . | K562/lp4 . | K562/lp3 + 6 . | K562/lp4 + 6 . | K562/lp3 + 4 . | K562/lp3 + 4 + 6 . | K562/lp6 . |
|---|---|---|---|---|---|---|---|---|---|
| Anti-c MS33 | 8.7 | 39.9 | 32.9 | 43.1 | 37.3 | 31.3 | 11.7 | 17.7 | 39.9 |
| Anti-c BS240 | 3.6 | 11.2 | 14.3 | 18.4 | 3.8 | 3.0 | 3.1 | 3.8 | 6.0 |
| Anti-c MS45 | 10.6 | 42.1 | 30.1 | 19.3 | 54.8 | 30.0 | 34.7 | 45.3 | 20.7 |
| Anti-c 951 | 8.4 | 16.1 | 5.2 | 6.8 | 7.3 | 5.3 | 3.3 | 3.7 | 10.3 |
| Anti-c MS40 | 4.4 | 10.0 | 3.7 | 3.3 | 4.9 | 4.6 | 3.5 | 3.7 | 5.3 |
| Anti-c MS51 | 9.1 | 42.1 | 27.8 | 34.7 | 30.8 | 20.7 | 10.8 | 14.1 | 24.4 |
| Anti-c MS37 | 6.4 | 30.0 | 16.0 | 32.7 | 52.0 | 24.1 | 10.6 | 11.2 | 19.2 |
| Anti-E 906 | 2.9 | 10.9 | 8.7 | 4.1 | 2.3 | 2.8 | 2.7 | 2.9 | 12.7 |
| Anti-E H98 | 3.9 | 26.0 | 2.8 | 2.8 | 2.5 | 2.9 | 2.8 | 3.0 | 2.7 |
| Anti-E H4-1 | 3.6 | 32.0 | 15.7 | 27.4 | 3.2 | 3.2 | 3.3 | 3.3 | 47.2 |
| Anti-E HIRO-18 | 3.6 | 50.1 | 22.5 | 40.8 | 3.6 | 4.3 | 3.6 | 4.2 | 41.1 |
Values in the table are expressed as the mean fluorescence intensity. Values twice that of background antibody binding (ie, that observed binding to a negative control K562 line) are considered positive and are shaded gray and boldfaced. The results indicate that some K562 SDM mutant clones exhibit partial c phenotypes in that they bind some anti-c but not all.