Summary of D Epitopes Expressed by Some Common Partial D Phenotypes and the K562 SDM Mutants
| Partial D . | Predicted External AA Altered . | Loop Involved . | EpD Present . | EpD Absent . |
|---|---|---|---|---|
| DII | 354 | loop6 | 1-5, 7-15, 17, 18, 19, 21, 22, 24, 12-22, 24, 27, 28, 3-37 | 6, 23, 25, 26, 29 |
| DIIIb | 103 | loop2 | 1-26; 28-37 | 27 |
| DIVa | 350 | loop3, loop6 | 6-15, 17-19, 21, 22, 25, 27, 28, 31-37 | 1-5, 23, 24, 26, 29, 30 |
| DIVb | 350, 353, 354 | loop6 | 7-15, 17-19, 21, 22, 25, 32, 33, 37 | 1-6, 23, 24, 26, 29, 30, 31, 34-36 |
| DVa | 223, 233 | loop4 | 3-6, 12-15, 17-19, 21-25, 27-30, 33-37 | 1, 2, 7-11, 26, 31, 32 |
| DVI | 169, 170, 172, 223, 233 | loop3, loop4 | 5, 6, 23-25, 30, 36, 37 | 1, 2, 3, 4, 7-22, 26-29, 31-35 |
| DVII | 110 | loop2 | 1-15, 17-19, 21, 23-26, 29, 30, 33-37 | 22, 27, 28, 31, 32 |
| DFR | 169, 170, 172 | loop3 | 1, 3, 5-8, 12-15, 23, 25, 29, 30, 34-37 | 2, 4, 9-11, 17-19, 21, 22, 24, 26-28, 31-33 |
| DBT | 223, 226, 233 350, 353, 354 | loop4 loop6 | 12, 13, 17, 18, 22, 33, 37 | 1-11, 14, 15, 19, 21, 23-32, 34-36 |
| RoHAR | 103, 169, 170, 172, 350, 353, 354 | loop2, 3, 6 | 7, 9, 12, 14, 17, 19 | 1-6, 8, 10, 11, 13, 15, 18, 21-37 |
| DMH | 1, 3-6, 10, 12, 13, 15, 17, 19, (20), 21, 23, 35-37 | 2, 7, 11, 18, (20), 22, 31, 32, 34 | ||
| HMi | 283 | loop5 | 1-3, 5-10, 12-15, 17-19, 23, 35-37 | 4, 11, 21, 22, 31-34 |
| K562/cE (loop3,4)D | 169, 170, 172, 223, 226, 233 | loop3, loop4 | (10), 11, 12, 13, 17, (18), 19, 21 | 1-7, (10), 13, 15, (18), 22-37 |
| K562/cE (loop4,6)D | 223, 226, 233 350, 353, 354 | loop4 loop6 | (5), 6, (12), (15), (17), (23), 30, (36) | 1, 3, (5), 7-11, (12), 13, (15), (17), 18-22, (23), 32, 33, 34, 35, (36), 37 |
| K562/cE (loop3,4,6) D | 169, 170, 172 223, 226, 233 350, 353, 354 | loop3 loop4 loop6 | 3-21, 23, 30, 34, 36, 37 | 1, 22, 32, 34 |
| K562/cE (loop 6)D | 350, 353, 354 | (5), (23) | 1-4, (5), 6-22, (23), 24-37 |
| Partial D . | Predicted External AA Altered . | Loop Involved . | EpD Present . | EpD Absent . |
|---|---|---|---|---|
| DII | 354 | loop6 | 1-5, 7-15, 17, 18, 19, 21, 22, 24, 12-22, 24, 27, 28, 3-37 | 6, 23, 25, 26, 29 |
| DIIIb | 103 | loop2 | 1-26; 28-37 | 27 |
| DIVa | 350 | loop3, loop6 | 6-15, 17-19, 21, 22, 25, 27, 28, 31-37 | 1-5, 23, 24, 26, 29, 30 |
| DIVb | 350, 353, 354 | loop6 | 7-15, 17-19, 21, 22, 25, 32, 33, 37 | 1-6, 23, 24, 26, 29, 30, 31, 34-36 |
| DVa | 223, 233 | loop4 | 3-6, 12-15, 17-19, 21-25, 27-30, 33-37 | 1, 2, 7-11, 26, 31, 32 |
| DVI | 169, 170, 172, 223, 233 | loop3, loop4 | 5, 6, 23-25, 30, 36, 37 | 1, 2, 3, 4, 7-22, 26-29, 31-35 |
| DVII | 110 | loop2 | 1-15, 17-19, 21, 23-26, 29, 30, 33-37 | 22, 27, 28, 31, 32 |
| DFR | 169, 170, 172 | loop3 | 1, 3, 5-8, 12-15, 23, 25, 29, 30, 34-37 | 2, 4, 9-11, 17-19, 21, 22, 24, 26-28, 31-33 |
| DBT | 223, 226, 233 350, 353, 354 | loop4 loop6 | 12, 13, 17, 18, 22, 33, 37 | 1-11, 14, 15, 19, 21, 23-32, 34-36 |
| RoHAR | 103, 169, 170, 172, 350, 353, 354 | loop2, 3, 6 | 7, 9, 12, 14, 17, 19 | 1-6, 8, 10, 11, 13, 15, 18, 21-37 |
| DMH | 1, 3-6, 10, 12, 13, 15, 17, 19, (20), 21, 23, 35-37 | 2, 7, 11, 18, (20), 22, 31, 32, 34 | ||
| HMi | 283 | loop5 | 1-3, 5-10, 12-15, 17-19, 23, 35-37 | 4, 11, 21, 22, 31-34 |
| K562/cE (loop3,4)D | 169, 170, 172, 223, 226, 233 | loop3, loop4 | (10), 11, 12, 13, 17, (18), 19, 21 | 1-7, (10), 13, 15, (18), 22-37 |
| K562/cE (loop4,6)D | 223, 226, 233 350, 353, 354 | loop4 loop6 | (5), 6, (12), (15), (17), (23), 30, (36) | 1, 3, (5), 7-11, (12), 13, (15), (17), 18-22, (23), 32, 33, 34, 35, (36), 37 |
| K562/cE (loop3,4,6) D | 169, 170, 172 223, 226, 233 350, 353, 354 | loop3 loop4 loop6 | 3-21, 23, 30, 34, 36, 37 | 1, 22, 32, 34 |
| K562/cE (loop 6)D | 350, 353, 354 | (5), (23) | 1-4, (5), 6-22, (23), 24-37 |
The table summarizes the reaction profiles of partial D phenotype red blood cells and the K562 lines described in this report using the 37 epitope model.14 Some antibodies of 1 epitope cluster fail to react with one partial D or K562 clone, whereas others react. These epitope clusters are indicated by parentheses.