The effect of CTX treatment on IFN-γ or IL-4 production and proliferative activity of spleen cells stimulated with T-Cell mitogens
| Treatment . | Stimuli* . | IFN-γ (ng/mL)† . | IL-4 (pg/mL) . | Proliferation (×10−3 cpm) . |
|---|---|---|---|---|
| None | <0.3 | <7 | 2.1 ± 0.5 | |
| PBS | Con A | 8.2 ± 0.6 | 73.7 ± 2.3 | 122.3 ± 16.1 |
| CD3 + IL-2 | 21.1 ± 1.9 | 198.4 ± 1.6 | 103.4 ± 11.5 | |
| None | <0.3 | <7 | 1.4 ± 0.2 | |
| CTX | Con A | 8.0 ± 0.0 | 99.2 ± 4.1‡ | 15.1 ± 4.4‡ |
| CD3 + IL-2 | 12.1 ± 0.0‡ | 203.9 ± 6.1 | 12.4 ± 5.1‡ |
| Treatment . | Stimuli* . | IFN-γ (ng/mL)† . | IL-4 (pg/mL) . | Proliferation (×10−3 cpm) . |
|---|---|---|---|---|
| None | <0.3 | <7 | 2.1 ± 0.5 | |
| PBS | Con A | 8.2 ± 0.6 | 73.7 ± 2.3 | 122.3 ± 16.1 |
| CD3 + IL-2 | 21.1 ± 1.9 | 198.4 ± 1.6 | 103.4 ± 11.5 | |
| None | <0.3 | <7 | 1.4 ± 0.2 | |
| CTX | Con A | 8.0 ± 0.0 | 99.2 ± 4.1‡ | 15.1 ± 4.4‡ |
| CD3 + IL-2 | 12.1 ± 0.0‡ | 203.9 ± 6.1 | 12.4 ± 5.1‡ |
CTX-SC were obtained 10 days after the last dose of CTX (100 mg/kg) was administered to mice.
Spleen cells were cultured in 96 flat-bottomed wells (0.4 × 106 cells/well) and stimulated at culture initiation with Con A (1 μg/mL) or anti-CD3 (2 μg/mL) plus IL-2 (50 units/mL).
Culture supernatants for cytokine quantification were recovered after 24 hours of culture. Proliferative activity was estimated by [3H]TdR incorporation after 72 hours of culture.
Values were significantly (P < .05) different from PBS-treated controls.