Multiplex PCR test primers for predicting Rh D and C/c phenotype and the presence of the RHD pseudogene
| Primer Name . | Sequence (5′ to 3′) . | Concentration (ng/μL) . | Product Size (bp) . |
|---|---|---|---|
| Exon7/for | AGCTCCATCATGGGCTACAA | 1 | 95 |
| Exon 7/rev | ATTGCCGGCTCCGACGGTATC | 1 | |
| Intron 3/for 1 | GGGTTGGGCTGGGTAAGCTCT | 1 | 498 or 535 |
| Intron 4/rev | GAACCTGCTCTGTGAAGTGCT | 4 | |
| Intron 3/for 2 | AACCTGGGAGGCAAATGTT | 10 | 250 |
| Exon 4 insert/rev | AATAAAACCCAGTAAGTTCATGTGG | 10 | |
| C/for | CAGGGCCACCACCATTTGAA | 12 | 320 |
| C/rev | GAACATGCCACTTCACTCCAG | 1.5 | |
| c/for | TCGGCCAAGATCTGACCG | 7 | 177 |
| c/rev | TGATGACCACCTTCCCAGG | 7 |
| Primer Name . | Sequence (5′ to 3′) . | Concentration (ng/μL) . | Product Size (bp) . |
|---|---|---|---|
| Exon7/for | AGCTCCATCATGGGCTACAA | 1 | 95 |
| Exon 7/rev | ATTGCCGGCTCCGACGGTATC | 1 | |
| Intron 3/for 1 | GGGTTGGGCTGGGTAAGCTCT | 1 | 498 or 535 |
| Intron 4/rev | GAACCTGCTCTGTGAAGTGCT | 4 | |
| Intron 3/for 2 | AACCTGGGAGGCAAATGTT | 10 | 250 |
| Exon 4 insert/rev | AATAAAACCCAGTAAGTTCATGTGG | 10 | |
| C/for | CAGGGCCACCACCATTTGAA | 12 | 320 |
| C/rev | GAACATGCCACTTCACTCCAG | 1.5 | |
| c/for | TCGGCCAAGATCTGACCG | 7 | 177 |
| c/rev | TGATGACCACCTTCCCAGG | 7 |