Oligonucleotide primers for amplifying whole exons or for identifying the presence of exons
| Exon . | Primer Name . | Direction . | Specificit . | Primer Position* . | Sequence (5′ to 3′) . |
|---|---|---|---|---|---|
| 1 | RH1F (rb13) | S | D + CE | Promoter −675 to −652 | CTAGAGCCAAACCCACATCTCCTT |
| RH1R (re11d) | AS | D + CE | Intron 1 129 to 106 | AGAAGATGGGGGAATCTTTTTCCT | |
| RHD1F (re01) | S | D | Promoter −149 to −132 | ATAGAGAGGCCAGCACAA | |
| RHIN1R | AS | D + CE | Intron 1 84 to 64 | TCTGTGCCCCTGGAGAACCAC | |
| 2 | RHEX2IN1F (re 13) | S | D + CE | Intron 1 −72 to −53 | ACTCTAATTTCATACCACCC |
| RH2R (re23) | AS | D + CE | Intron 2 250 to 227 | AAAGGATGCAGGAGGAATGTAGGC | |
| 3 | RH3F (ra21) | S | D + CE | Intron 2 2823 to 2842 | GTGCCACTTGACTTGGGACT |
| RHDIN2F (rb20d) | S | D | Intron 2 −25 to −8 | TCCTGGCTCTCCCTCTCT | |
| RH3R (rb21) | AS | D + CE | Intron 3 28 to 11 | AGGTCCCTCCTCCAGCAC | |
| RHDEX3F | S | D | Exon 3 364 to 383 | TCGGTGCTGATCTCAGTGGA | |
| RHDEX3R | AS | D | Exon 3 474 to 455 | ACTGATGACCATCCTCAGGT | |
| 4 | RHDIN3F | S | D | Intron 3 −36 to −16 | GCCGACACTCACTGCTCTTAC |
| RHD4R (rb12) | AS | D | Intron 4 197 to 174 | TCCTGAACCTGCTCTGTGAAGTGC | |
| RHDEX4F | S | D | Exon 4 582 to 602 | AACGGAGGATAAAGATCAGAC | |
| RHDIN4R | AS | D | Intron 4 193 to 173 | GAACCTGCTCTGTGAAGTGCT | |
| 5 | RHD5F (rb11) | S | D | Intron 4 −267 to −243 | TACCTTTGAATTAAGCACTTCACAG |
| RHIN5R | AS | D + CE | Intron 5 73 to 56 | GTGGGGAGGGGCATAAAT | |
| RHDMULTI5F | S | D | Exon 5 648 to 667 | GTGGATGTTCTGGCCAAGTT | |
| RHDMULTI5R | AS | D | Intron 6 16 to exon 5 799 | GCAGCGCCCTGCTCACCTT | |
| 6 | RH6F (rf51 | S | D + CE | Intron 5 −332 to −314 | CAAAAACCCATTCTTCCCG |
| RHDIN6R | AS | D | Intron 6 41 to 21 | CTTCAGCCAAAGCAGAGGAGG | |
| RHEX6F | S | D + CE | Exon 6 898 to 916 | GTGGCTGGGCTGATCTACG | |
| RHDEX6R | AS | D | Intron 7 15 to exon 6 932 | TGTCTAGTTTCTTACCGGCAAGT | |
| RHDΨEX6R | AS | DΨ | Exon 6 824 to 807 | AACACCGCACTGTGCTCC | |
| 7 | RHDIN6F (re621) | S | D | Intron 6 −102 to −85 | CATCCCCCTTTGGTGGCC |
| RHDIN7R (re75) | AS | D | Intron 7 168 to 151 | AAGGTAGGGGCTGGACAG | |
| RHEX7F2 | S | D + CE | Exon 7 949 to 967 | AACCGAGTGCTGGGGATTC | |
| RHDEX7R | AS | D | Exon 7 1059 to intron 8 7 | ACCCACATGCCATTGCCGGCT | |
| 9 | RHD9F | S | D | Exon 9 1154 to 1170 | AACAGGTTTGCTCCTAAATCTT |
| RHEX9R | AS | D | Exon 9 1219 to 1193 | AAACTTGGTCATCAAAATATTTAACCT | |
| 10 | RH10F (re91) | S | D + CE | Intron 9 −67 to −45 | CAAGAGATCAAGCCAAAATCAGT |
| RHD10R (rr4) | AS | D | 3′ UTR 1541 to 1522 | AGCTTACTGGATGACCACCA |
| Exon . | Primer Name . | Direction . | Specificit . | Primer Position* . | Sequence (5′ to 3′) . |
|---|---|---|---|---|---|
| 1 | RH1F (rb13) | S | D + CE | Promoter −675 to −652 | CTAGAGCCAAACCCACATCTCCTT |
| RH1R (re11d) | AS | D + CE | Intron 1 129 to 106 | AGAAGATGGGGGAATCTTTTTCCT | |
| RHD1F (re01) | S | D | Promoter −149 to −132 | ATAGAGAGGCCAGCACAA | |
| RHIN1R | AS | D + CE | Intron 1 84 to 64 | TCTGTGCCCCTGGAGAACCAC | |
| 2 | RHEX2IN1F (re 13) | S | D + CE | Intron 1 −72 to −53 | ACTCTAATTTCATACCACCC |
| RH2R (re23) | AS | D + CE | Intron 2 250 to 227 | AAAGGATGCAGGAGGAATGTAGGC | |
| 3 | RH3F (ra21) | S | D + CE | Intron 2 2823 to 2842 | GTGCCACTTGACTTGGGACT |
| RHDIN2F (rb20d) | S | D | Intron 2 −25 to −8 | TCCTGGCTCTCCCTCTCT | |
| RH3R (rb21) | AS | D + CE | Intron 3 28 to 11 | AGGTCCCTCCTCCAGCAC | |
| RHDEX3F | S | D | Exon 3 364 to 383 | TCGGTGCTGATCTCAGTGGA | |
| RHDEX3R | AS | D | Exon 3 474 to 455 | ACTGATGACCATCCTCAGGT | |
| 4 | RHDIN3F | S | D | Intron 3 −36 to −16 | GCCGACACTCACTGCTCTTAC |
| RHD4R (rb12) | AS | D | Intron 4 197 to 174 | TCCTGAACCTGCTCTGTGAAGTGC | |
| RHDEX4F | S | D | Exon 4 582 to 602 | AACGGAGGATAAAGATCAGAC | |
| RHDIN4R | AS | D | Intron 4 193 to 173 | GAACCTGCTCTGTGAAGTGCT | |
| 5 | RHD5F (rb11) | S | D | Intron 4 −267 to −243 | TACCTTTGAATTAAGCACTTCACAG |
| RHIN5R | AS | D + CE | Intron 5 73 to 56 | GTGGGGAGGGGCATAAAT | |
| RHDMULTI5F | S | D | Exon 5 648 to 667 | GTGGATGTTCTGGCCAAGTT | |
| RHDMULTI5R | AS | D | Intron 6 16 to exon 5 799 | GCAGCGCCCTGCTCACCTT | |
| 6 | RH6F (rf51 | S | D + CE | Intron 5 −332 to −314 | CAAAAACCCATTCTTCCCG |
| RHDIN6R | AS | D | Intron 6 41 to 21 | CTTCAGCCAAAGCAGAGGAGG | |
| RHEX6F | S | D + CE | Exon 6 898 to 916 | GTGGCTGGGCTGATCTACG | |
| RHDEX6R | AS | D | Intron 7 15 to exon 6 932 | TGTCTAGTTTCTTACCGGCAAGT | |
| RHDΨEX6R | AS | DΨ | Exon 6 824 to 807 | AACACCGCACTGTGCTCC | |
| 7 | RHDIN6F (re621) | S | D | Intron 6 −102 to −85 | CATCCCCCTTTGGTGGCC |
| RHDIN7R (re75) | AS | D | Intron 7 168 to 151 | AAGGTAGGGGCTGGACAG | |
| RHEX7F2 | S | D + CE | Exon 7 949 to 967 | AACCGAGTGCTGGGGATTC | |
| RHDEX7R | AS | D | Exon 7 1059 to intron 8 7 | ACCCACATGCCATTGCCGGCT | |
| 9 | RHD9F | S | D | Exon 9 1154 to 1170 | AACAGGTTTGCTCCTAAATCTT |
| RHEX9R | AS | D | Exon 9 1219 to 1193 | AAACTTGGTCATCAAAATATTTAACCT | |
| 10 | RH10F (re91) | S | D + CE | Intron 9 −67 to −45 | CAAGAGATCAAGCCAAAATCAGT |
| RHD10R (rr4) | AS | D | 3′ UTR 1541 to 1522 | AGCTTACTGGATGACCACCA |
Indicates that numbers for exon positions represent nucleotide position in cDNA. Numbers for introns are relative to adjacent exons. Primer names in parentheses are as described by Wagner et al.21 Introduced mismatch in RHDΨEX6R is underlined.