15-R/S-methyl-LXA4 Does Not Alter E-Selectin and ICAM-1 Expression on Human Coronary Artery Endothelial Cells
| . | 15-R/S- methyl-LXA4 . | E-Selectin (RFI) . | ICAM-1 (RFI) . |
|---|---|---|---|
| Control | 0 | 2 ± 1 | 140 ± 31 |
| LPS | 0 | 50 ± 5 | 327 ± 41 |
| LPS | 5 nmol/L | 51 ± 5 | 324 ± 31 |
| LPS | 50 nmol/L | 49 ± 4 | 305 ± 21 |
| LPS | 500 nmol/L | 45 ± 4 | 296 ± 27 |
| LPS | 5,000 nmol/L | 45 ± 2 | 302 ± 21 |
| TNF-α | 0 | 24 ± 3 | 386 ± 27 |
| TNF-α | 500 nmol/L | 19 ± 2 | 335 ± 32 |
| TNF-α | 5,000 nmol/L | 20 ± 1 | 357 ± 12 |
| . | 15-R/S- methyl-LXA4 . | E-Selectin (RFI) . | ICAM-1 (RFI) . |
|---|---|---|---|
| Control | 0 | 2 ± 1 | 140 ± 31 |
| LPS | 0 | 50 ± 5 | 327 ± 41 |
| LPS | 5 nmol/L | 51 ± 5 | 324 ± 31 |
| LPS | 50 nmol/L | 49 ± 4 | 305 ± 21 |
| LPS | 500 nmol/L | 45 ± 4 | 296 ± 27 |
| LPS | 5,000 nmol/L | 45 ± 2 | 302 ± 21 |
| TNF-α | 0 | 24 ± 3 | 386 ± 27 |
| TNF-α | 500 nmol/L | 19 ± 2 | 335 ± 32 |
| TNF-α | 5,000 nmol/L | 20 ± 1 | 357 ± 12 |
Confluent HCAEC monolayers were left unstimulated (control) or challenged with 1 μg/mL LPS or 2.5 ng/mL TNF-α for 4 hours at 37°C in the presence of 15-R/S-methyl-LXA4. Values are the mean ± SEM of 3 independent experiments.
Abbreviation: RFI, relative fluorescence intensity.