Effect of FCS-Containing Medium on Expansion of ELTC-CFC
| Experiment No. . | Fold Cell Expansion . | 12-wk LTC-CFC, % of Control . | ||
|---|---|---|---|---|
| SF . | 20% FCS . | SF . | 20% FCS . | |
| 1 | 14 | 17 | 269 | 7 |
| 2 | 7 | 8 | 137 | 29 |
| 3 | 42 | 44 | 3,002 | 394 |
| 4 | 19 | 13 | 2,811 | 211 |
| Experiment No. . | Fold Cell Expansion . | 12-wk LTC-CFC, % of Control . | ||
|---|---|---|---|---|
| SF . | 20% FCS . | SF . | 20% FCS . | |
| 1 | 14 | 17 | 269 | 7 |
| 2 | 7 | 8 | 137 | 29 |
| 3 | 42 | 44 | 3,002 | 394 |
| 4 | 19 | 13 | 2,811 | 211 |
One thousand CD34+CD38− cells were cultured in FCS-containing or in SF-medium supplemented with KFM at 250 ng/mL each for 12 days. As a control, the same number of freshly isolated CD34+CD38− cells was transferred directly to pre-established irradiated stroma. After 12 weeks of stroma cocultivation, the number of CFC produced was established. Results are the means of triplicate wells per group.