Prolonged Exposure to KL + IL-3 + IL-6, But Not KL + FL + MGDF, Deplete ELTC-CFC
| Experiment No. . | Cytokines . | 12-wk LTC-CFC, % of Control . | |
|---|---|---|---|
| d7 . | d12 . | ||
| 1 | K36 | 9 (16) | 3 (5) |
| KF3 | 171 (28) | 291 (267) | |
| KFM | 529 (193) | 1,568 (268) | |
| 2 | K36 | 16 (15) | 33 (19) |
| KF3 | 202 (147) | 68 (84) | |
| KFM | 44 (38) | 269 (88) | |
| 3 | K36 | 17 (30) | 53 (91) |
| KF3 | 156 (136) | 158 (80) | |
| KFM | 64 (50) | 777 (874) | |
| 4 | K36 | 0 | 0 |
| KF3 | 1,217 (507) | 2,136 (1,176) | |
| KFM | 1,589 (404) | 3,002 (1,331) | |
| Experiment No. . | Cytokines . | 12-wk LTC-CFC, % of Control . | |
|---|---|---|---|
| d7 . | d12 . | ||
| 1 | K36 | 9 (16) | 3 (5) |
| KF3 | 171 (28) | 291 (267) | |
| KFM | 529 (193) | 1,568 (268) | |
| 2 | K36 | 16 (15) | 33 (19) |
| KF3 | 202 (147) | 68 (84) | |
| KFM | 44 (38) | 269 (88) | |
| 3 | K36 | 17 (30) | 53 (91) |
| KF3 | 156 (136) | 158 (80) | |
| KFM | 64 (50) | 777 (874) | |
| 4 | K36 | 0 | 0 |
| KF3 | 1,217 (507) | 2,136 (1,176) | |
| KFM | 1,589 (404) | 3,002 (1,331) | |
One thousand to 1,500 human CD34+CD38−cells were cultured in SF-medium in the presence of the indicated cytokines. As a control for the content of ELTC-IC in freshly isolated CD34+CD38− cells, the same number of cells was transferred directly to pre-established irradiated stroma. After 12 weeks of stroma cocultivation, the number of CFC produced was evaluated in methylcellulose cultures. Results are presented as the mean (SD) of triplicate wells per group and time point. Controls (fresh, nonexpanded CD34+CD38− cells) were set to represent 100% and reflected 55 ± 7 colonies in experiment no. 1, 40 ± 15 colonies in experiment no. 2, 11 ± 10 colonies in experiment no. 3, and 9 ± 5 colonies in experiment no. 4.