KL + IL-3 + IL-6 as Well as KL + FL + MGDF Expand 5-Week LTC-CFC
| Experiment No. . | Cytokines . | 5-wk LTC-CFC, % of Control . | |
|---|---|---|---|
| d7 . | d12 . | ||
| 1 | K36 | 360 (55) | 176 (62) |
| KF3 | 448 (324) | 248 (234) | |
| KFM | 3,522 (396) | 2,070 (1,647) | |
| 2 | K36 | 952 (829) | 344 (242) |
| KF3 | 3,254 (1,470) | 5,026 (1,957) | |
| KFM | 8,862 (1,604) | 8,069 (458) | |
| 3 | K36 | 5,889 (2,143) | 816 (408) |
| KF3 | 21,778 (5,551) | 6,186 (652) | |
| KFM | 24,384 (11,729) | 18,333 (7,638) | |
| Experiment No. . | Cytokines . | 5-wk LTC-CFC, % of Control . | |
|---|---|---|---|
| d7 . | d12 . | ||
| 1 | K36 | 360 (55) | 176 (62) |
| KF3 | 448 (324) | 248 (234) | |
| KFM | 3,522 (396) | 2,070 (1,647) | |
| 2 | K36 | 952 (829) | 344 (242) |
| KF3 | 3,254 (1,470) | 5,026 (1,957) | |
| KFM | 8,862 (1,604) | 8,069 (458) | |
| 3 | K36 | 5,889 (2,143) | 816 (408) |
| KF3 | 21,778 (5,551) | 6,186 (652) | |
| KFM | 24,384 (11,729) | 18,333 (7,638) | |
One hundred fifty human CD34+CD38− cells were cultured in SF-medium in the presence of the indicated cytokines. As a control for the content of 5-week LTC-CFC in freshly isolated CD34+CD38− cells, the same number of cells was transferred directly to pre-established irradiated stroma. After 5 weeks of stroma cocultivation, the number of CFC produced was evaluated in methylcellulose cultures. Results are presented as the mean (SD) of triplicate wells per group and time point. Controls (fresh, nonexpanded CD34+CD38− cells) were set to represent 100% and reflected 253 ± 6 colonies in experiment no. 1, 19 ± 13 colonies in experiment no. 2, and 5 ± 2 colonies in experiment no. 3.