Binding of IRF Complexes to Mutated ISG15 ISRE Oligonucleotides
Oligo . | Sequence . | Binding* . | ||||
---|---|---|---|---|---|---|
B Lymphocyte . | Macrophage . | |||||
ICSBP . | IRF-2 . | IRF-4 . | ICSBP . | IRF-4 . | ||
ISRE | GATCCTCGGGAAAGGGAAACCGAAACTGAAGCC | ++ | ++ | ++ | ++ | ++ |
M1 | GATCCTCGGGAAAGGGcgtCCGAAACTGAAGCC | − | − | − | − | − |
M2 | GATCCTCGGGcgtGGGcgtCCGAAACTGAAGCC | − | − | − | − | − |
M3 | GATCCTCGGGcgtGGGcgtCCGcgtCTGAAGCC | − | − | − | − | − |
M4 | GATCCTCGGGcgtGGGAAACCGAAACTGAAGCC | + | + | + | + | + |
M5 | GATCCTCGGGAAAGGGAAACCGcgtCTGAAGCC | − | − | − | − | − |
GG1 | GATCCTCGGGAAAGGggGAAACCGAAACTGAAGCC | + | + | − | + | + |
GG2 | GATCCTCGGGAAAGGGAAACCggGAAACTGAAGCC | − | − | − | − | − |
Oligo . | Sequence . | Binding* . | ||||
---|---|---|---|---|---|---|
B Lymphocyte . | Macrophage . | |||||
ICSBP . | IRF-2 . | IRF-4 . | ICSBP . | IRF-4 . | ||
ISRE | GATCCTCGGGAAAGGGAAACCGAAACTGAAGCC | ++ | ++ | ++ | ++ | ++ |
M1 | GATCCTCGGGAAAGGGcgtCCGAAACTGAAGCC | − | − | − | − | − |
M2 | GATCCTCGGGcgtGGGcgtCCGAAACTGAAGCC | − | − | − | − | − |
M3 | GATCCTCGGGcgtGGGcgtCCGcgtCTGAAGCC | − | − | − | − | − |
M4 | GATCCTCGGGcgtGGGAAACCGAAACTGAAGCC | + | + | + | + | + |
M5 | GATCCTCGGGAAAGGGAAACCGcgtCTGAAGCC | − | − | − | − | − |
GG1 | GATCCTCGGGAAAGGggGAAACCGAAACTGAAGCC | + | + | − | + | + |
GG2 | GATCCTCGGGAAAGGGAAACCggGAAACTGAAGCC | − | − | − | − | − |
Abbreviations: ++, strong binding; +, weak binding; −, no binding.
The presence of individual IRF proteins was concluded from positive supershifts with the corresponding antibody. The mobility of protein complexes formed with mutated oligonucleotides is in part altered.