Table 1.

Binding of IRF Complexes to Mutated ISG15 ISRE Oligonucleotides

Oligo Sequence Binding*
B Lymphocyte Macrophage
ICSBP IRF-2 IRF-4 ICSBP IRF-4
ISRE GATCCTCGGGAAAGGGAAACCGAAACTGAAGCC  ++  ++  ++ ++  ++  
M1  GATCCTCGGGAAAGGGcgtCCGAAACTGAAGCC −  −  −  −  −  
M2 GATCCTCGGGcgtGGGcgtCCGAAACTGAAGCC  −  − −  −  −  
M3 GATCCTCGGGcgtGGGcgtCCGcgtCTGAAGCC  −  − −  −  −  
M4 GATCCTCGGGcgtGGGAAACCGAAACTGAAGCC  +  +  +  +  
M5  GATCCTCGGGAAAGGGAAACCGcgtCTGAAGCC −  −  −  −  −  
GG1 GATCCTCGGGAAAGGggGAAACCGAAACTGAAGCC  +  +  − +  +  
GG2  GATCCTCGGGAAAGGGAAACCggGAAACTGAAGCC −  −  −  −  − 
Oligo Sequence Binding*
B Lymphocyte Macrophage
ICSBP IRF-2 IRF-4 ICSBP IRF-4
ISRE GATCCTCGGGAAAGGGAAACCGAAACTGAAGCC  ++  ++  ++ ++  ++  
M1  GATCCTCGGGAAAGGGcgtCCGAAACTGAAGCC −  −  −  −  −  
M2 GATCCTCGGGcgtGGGcgtCCGAAACTGAAGCC  −  − −  −  −  
M3 GATCCTCGGGcgtGGGcgtCCGcgtCTGAAGCC  −  − −  −  −  
M4 GATCCTCGGGcgtGGGAAACCGAAACTGAAGCC  +  +  +  +  
M5  GATCCTCGGGAAAGGGAAACCGcgtCTGAAGCC −  −  −  −  −  
GG1 GATCCTCGGGAAAGGggGAAACCGAAACTGAAGCC  +  +  − +  +  
GG2  GATCCTCGGGAAAGGGAAACCggGAAACTGAAGCC −  −  −  −  − 

Abbreviations: ++, strong binding; +, weak binding; −, no binding.

*

The presence of individual IRF proteins was concluded from positive supershifts with the corresponding antibody. The mobility of protein complexes formed with mutated oligonucleotides is in part altered.

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