Inhibition of vWF-Dependent CHO-GPIbβ/IX Cell Aggregation
| Inhibitor . | CHO-GPIbαβ/IX Aggregation (%) . | ||
|---|---|---|---|
| Buffer . | Ristocetin . | Heparin . | |
| None | 2.2 ± 0.5 | 60.3 ± 5.8 | 50.2 ± 4 |
| Trypsin | 2.9 ± 0.9 | 0.9 ± 0.1* | 3.3 ± 1.9* |
| Elastase | 3.5 ± 0.9 | 3 ± 0.5* | 4.3 ± 2.2* |
| Mocarhagin | 2.6 ± 1.0 | 1 ± 1* | 7.5 ± 4.5† |
| Inhibitor . | CHO-GPIbαβ/IX Aggregation (%) . | ||
|---|---|---|---|
| Buffer . | Ristocetin . | Heparin . | |
| None | 2.2 ± 0.5 | 60.3 ± 5.8 | 50.2 ± 4 |
| Trypsin | 2.9 ± 0.9 | 0.9 ± 0.1* | 3.3 ± 1.9* |
| Elastase | 3.5 ± 0.9 | 3 ± 0.5* | 4.3 ± 2.2* |
| Mocarhagin | 2.6 ± 1.0 | 1 ± 1* | 7.5 ± 4.5† |
CHO-GPIbαβ/IX cell aggregation was assessed at 6 cycles per second in the presence of 10 nmol/L vWF and either buffer, 1.4 mg/mL ristocetin or 100 μg/mL heparin. Cells were treated with trypsin (50 μg/mL), elastase (200 nmol/L), or mocarhagin (10 μg/mL). The percentage of aggregated cells was calculated and the mean ± SEM was determined in 3 separate experiments. Statistical significance of differences between means was evaluated using the Student'st-test for paired samples by comparing aggregation in the presence of enzyme versus the control corresponding sample in the absence of enzyme.
P < .0025.
P < .05.