Table 4.

Results of Proliferation of Individual CD34+/Kit+ Cells

SF, FL, IL-3SF, FL, IL-3, G-CSF
No. of Clones Proliferated Mean No.of Cells Per Each Clone No. of Clones Proliferated Mean No.of Cells Per Each Clone
Patient 1   4  125  18  206  
Patient 2   6  130  22  250  
Control 1  28  191  61 609  
Control 2  20  203  69  828  
Control 3 15  173  90  770  
Control 4  15  185  51 780  
Control 5  28  215  69  828 
SF, FL, IL-3SF, FL, IL-3, G-CSF
No. of Clones Proliferated Mean No.of Cells Per Each Clone No. of Clones Proliferated Mean No.of Cells Per Each Clone
Patient 1   4  125  18  206  
Patient 2   6  130  22  250  
Control 1  28  191  61 609  
Control 2  20  203  69  828  
Control 3 15  173  90  770  
Control 4  15  185  51 780  
Control 5  28  215  69  828 

Two hundred sixteen CD34+/Kit+ cells were individually seeded in each well of 72-well plates containing serum-deprived media in the presence of SF, FL, and IL-3 with or without G-CSF and cultured for 10 days. Data represent the number of clones proliferated and the mean number of cells per each proliferated clone.

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