Results of Proliferation of Individual CD34+/Kit+ Cells
. | SF, FL, IL-3 . | SF, FL, IL-3, G-CSF . | ||
---|---|---|---|---|
No. of Clones Proliferated . | Mean No.of Cells Per Each Clone . | No. of Clones Proliferated . | Mean No.of Cells Per Each Clone . | |
Patient 1 | 4 | 125 | 18 | 206 |
Patient 2 | 6 | 130 | 22 | 250 |
Control 1 | 28 | 191 | 61 | 609 |
Control 2 | 20 | 203 | 69 | 828 |
Control 3 | 15 | 173 | 90 | 770 |
Control 4 | 15 | 185 | 51 | 780 |
Control 5 | 28 | 215 | 69 | 828 |
. | SF, FL, IL-3 . | SF, FL, IL-3, G-CSF . | ||
---|---|---|---|---|
No. of Clones Proliferated . | Mean No.of Cells Per Each Clone . | No. of Clones Proliferated . | Mean No.of Cells Per Each Clone . | |
Patient 1 | 4 | 125 | 18 | 206 |
Patient 2 | 6 | 130 | 22 | 250 |
Control 1 | 28 | 191 | 61 | 609 |
Control 2 | 20 | 203 | 69 | 828 |
Control 3 | 15 | 173 | 90 | 770 |
Control 4 | 15 | 185 | 51 | 780 |
Control 5 | 28 | 215 | 69 | 828 |
Two hundred sixteen CD34+/Kit+ cells were individually seeded in each well of 72-well plates containing serum-deprived media in the presence of SF, FL, and IL-3 with or without G-CSF and cultured for 10 days. Data represent the number of clones proliferated and the mean number of cells per each proliferated clone.