Formation of GM Colonies of CD34+/c-Kit+ Cells Supported With Various Factors
| Factors . | No. of GM Colonies . | |||||
|---|---|---|---|---|---|---|
| Patient 1 . | Patient 2 . | Patient 3 . | Patient 4 . | Normal Subjects (Mean ± SD, n = 6) . | Normal Subjects (Range, n = 6) . | |
| G-CSF | 2 | 1 | 3 | 2 | 14 ± 8 | 5-22 |
| IL-3 | 1 | 2 | 1 | NT | 11 ± 6 | 4-20 |
| SF | 2 | 0 | 0 | NT | 0 | 0 |
| FL | 1 | 1 | 0 | NT | 2 ± 2 | 2-4 |
| IL-3, G-CSF | 4 | 5 | 20 | 18 | 30 ± 12 | 20-49 |
| SF, G-CSF | 12 | 4 | 17 | 15 | 31 ± 12 | 16-43 |
| FL, G-CSF | 9 | 2 | 17 | 16 | 27 ± 12 | 16-44 |
| SF, FL, IL-3 | 8 | 19 | 25 | 22 | 52 ± 14 | 38-77 |
| SF, FL, IL-3, G-CSF | 22 | 20 | 62 | 65 | 100 ± 30 | 76-164 |
| Factors . | No. of GM Colonies . | |||||
|---|---|---|---|---|---|---|
| Patient 1 . | Patient 2 . | Patient 3 . | Patient 4 . | Normal Subjects (Mean ± SD, n = 6) . | Normal Subjects (Range, n = 6) . | |
| G-CSF | 2 | 1 | 3 | 2 | 14 ± 8 | 5-22 |
| IL-3 | 1 | 2 | 1 | NT | 11 ± 6 | 4-20 |
| SF | 2 | 0 | 0 | NT | 0 | 0 |
| FL | 1 | 1 | 0 | NT | 2 ± 2 | 2-4 |
| IL-3, G-CSF | 4 | 5 | 20 | 18 | 30 ± 12 | 20-49 |
| SF, G-CSF | 12 | 4 | 17 | 15 | 31 ± 12 | 16-43 |
| FL, G-CSF | 9 | 2 | 17 | 16 | 27 ± 12 | 16-44 |
| SF, FL, IL-3 | 8 | 19 | 25 | 22 | 52 ± 14 | 38-77 |
| SF, FL, IL-3, G-CSF | 22 | 20 | 62 | 65 | 100 ± 30 | 76-164 |
Cultures were performed in serum-deprived conditions containing 500 CD34+/c-Kit+ cells and designated factors. Data of the patients represent the mean of triplicate cultures.
Abbreviation: NT, not tested.