Formation of GM Colonies of Bone Marrow Mononuclear Cells Supported With Various Factors
| Factors . | No. of GM Colonies . | |||||
|---|---|---|---|---|---|---|
| Patient 1 . | Patient 2 . | Patient 3 . | Patient 4 . | Normal Subjects (Mean ± SD, n = 6) . | Normal Subjects (Range, n = 6) . | |
| G-CSF | 4 | 18 | 8 | 6 | 38 ± 14 | 24-48 |
| IL-3 | 2 | 5 | 6 | 7 | 9 ± 4 | 5-15 |
| SF | 1 | 0 | 0 | 0 | 0 ± 1 | 0-1 |
| FL | 4 | 3 | 1 | 1 | 4 ± 5 | 1-12 |
| IL-3, G-CSF | 9 | 26 | 16 | 20 | 50 ± 18 | 28-73 |
| SF, G-CSF | 11 | 24 | 18 | 16 | 39 ± 17 | 25-58 |
| FL, G-CSF | 9 | 26 | 12 | 11 | 33 ± 9 | 20-42 |
| SF, FL, IL-3 | 12 | 36 | 14 | 20 | 51 ± 21 | 32-82 |
| SF, FL, IL-3, G-CSF | 18 | 50 | 36 | 40 | 99 ± 30 | 64-131 |
| Factors . | No. of GM Colonies . | |||||
|---|---|---|---|---|---|---|
| Patient 1 . | Patient 2 . | Patient 3 . | Patient 4 . | Normal Subjects (Mean ± SD, n = 6) . | Normal Subjects (Range, n = 6) . | |
| G-CSF | 4 | 18 | 8 | 6 | 38 ± 14 | 24-48 |
| IL-3 | 2 | 5 | 6 | 7 | 9 ± 4 | 5-15 |
| SF | 1 | 0 | 0 | 0 | 0 ± 1 | 0-1 |
| FL | 4 | 3 | 1 | 1 | 4 ± 5 | 1-12 |
| IL-3, G-CSF | 9 | 26 | 16 | 20 | 50 ± 18 | 28-73 |
| SF, G-CSF | 11 | 24 | 18 | 16 | 39 ± 17 | 25-58 |
| FL, G-CSF | 9 | 26 | 12 | 11 | 33 ± 9 | 20-42 |
| SF, FL, IL-3 | 12 | 36 | 14 | 20 | 51 ± 21 | 32-82 |
| SF, FL, IL-3, G-CSF | 18 | 50 | 36 | 40 | 99 ± 30 | 64-131 |
Cultures were performed in serum-deprived conditions containing 2 × 104 nonadherent LDBMC and designated factors. Data of the patients represent the mean of triplicate cultures.