Percentage of Reticulocytes Within PIGA(+) and PIGA(−) RBCs in Lox-▵Piga-lacZ Mice and the Calculated Half-Life of PIGA(−) RBCs
| Mouse ID . | PIGA(−) RBC % . | % Retic Within PIGA(+) RBCs . | % Retic Within PIGA(−) RBCs . | t1/2 of PIGA(−) RBCs (d) . |
|---|---|---|---|---|
| 1.3 | 12.2 | 4.8 | 8.8 | 8.2 |
| 12.21 | 9.5 | 3.9 | 8.9 | 6.6 |
| 15.1 | 9.5 | 4.6 | 13.1 | 5.3 |
| 406.5 | 13.1 | 4.5 | 7.9 | 8.5 |
| 406.12 | 10.1 | 6.1 | 15.1 | 6.1 |
| 20.4 | 8.6 | 3.9 | 10 | 5.9 |
| 28.5 | 7.1 | 3.4 | 4.6 | 11.1 |
| 6.1 | 21.3 | 6.1 | 7.7 | 11.9 |
| 12.2 | 5.5 | 6 | 14.6 | 6.2 |
| 11.2 | 6.4 | 3 | 7.6 | 5.9 |
| 12.11 | 7.6 | 4.6 | 13 | 5.3 |
| Mean | 10.1 | 4.6 | 10.1 | 7.3 |
| Controls | t1/2 of PIGA(+) RBC | |||
| C 1.45 | 0.1 | 5.1 | 0 | 154-150 |
| C 15 | 0 | 3.9 | 0 | 154-150 |
| Mouse ID . | PIGA(−) RBC % . | % Retic Within PIGA(+) RBCs . | % Retic Within PIGA(−) RBCs . | t1/2 of PIGA(−) RBCs (d) . |
|---|---|---|---|---|
| 1.3 | 12.2 | 4.8 | 8.8 | 8.2 |
| 12.21 | 9.5 | 3.9 | 8.9 | 6.6 |
| 15.1 | 9.5 | 4.6 | 13.1 | 5.3 |
| 406.5 | 13.1 | 4.5 | 7.9 | 8.5 |
| 406.12 | 10.1 | 6.1 | 15.1 | 6.1 |
| 20.4 | 8.6 | 3.9 | 10 | 5.9 |
| 28.5 | 7.1 | 3.4 | 4.6 | 11.1 |
| 6.1 | 21.3 | 6.1 | 7.7 | 11.9 |
| 12.2 | 5.5 | 6 | 14.6 | 6.2 |
| 11.2 | 6.4 | 3 | 7.6 | 5.9 |
| 12.11 | 7.6 | 4.6 | 13 | 5.3 |
| Mean | 10.1 | 4.6 | 10.1 | 7.3 |
| Controls | t1/2 of PIGA(+) RBC | |||
| C 1.45 | 0.1 | 5.1 | 0 | 154-150 |
| C 15 | 0 | 3.9 | 0 | 154-150 |
Abbreviations: PMN, granulocytes; retic, reticulocytes.
t1/2 of normal murine RBCs = 15 days.25