Proportion of PIGA(−) Blood Cells in Peripheral Blood in PIGA(−) Mosaic Mice
| Age (wk) . | PIGA(−) RBC % (n) . | PIGA(−) PMN % (n) . | PIGA(−) T Cells % (n) . | PIGA(−) B Cells % (n) . | PIGA(−) CD4 Cells % (n) . | PIGA(−) CD8 Cells % (n) . |
|---|---|---|---|---|---|---|
| Birth | 17.8 (32†) | ND | ND | ND | ND | ND |
| 4 | 8.4 (19†) | 12.2 (37) | 16.3 (7‡) | 6 (7‡) | ND | ND |
| P < .05 | ||||||
| 16 | 4 (39) | 11.2 (39) | 18 (39) | 8.6 (39) | 16.8 (37) | 16.9 (39) |
| P = NS | P = NS | P = NS | P < .05 | |||
| 32 | 4.1 (38*) | 10.5 (37) | 18.6 (37) | 11 (37) | 13.3 (37) | 20.1 (37) |
| P = NS | P = NS | P = NS | P < .05 | P < .05 | P < .05 | |
| 52 | 3 (37*) | 10.5 (37) | 18.8 (37) | 7.6 (35) | 13.5 (37) | 24.5 (36) |
| P = NS | P = NS | P = NS | P < .05 | P < .05 | P < .05 |
| Age (wk) . | PIGA(−) RBC % (n) . | PIGA(−) PMN % (n) . | PIGA(−) T Cells % (n) . | PIGA(−) B Cells % (n) . | PIGA(−) CD4 Cells % (n) . | PIGA(−) CD8 Cells % (n) . |
|---|---|---|---|---|---|---|
| Birth | 17.8 (32†) | ND | ND | ND | ND | ND |
| 4 | 8.4 (19†) | 12.2 (37) | 16.3 (7‡) | 6 (7‡) | ND | ND |
| P < .05 | ||||||
| 16 | 4 (39) | 11.2 (39) | 18 (39) | 8.6 (39) | 16.8 (37) | 16.9 (39) |
| P = NS | P = NS | P = NS | P < .05 | |||
| 32 | 4.1 (38*) | 10.5 (37) | 18.6 (37) | 11 (37) | 13.3 (37) | 20.1 (37) |
| P = NS | P = NS | P = NS | P < .05 | P < .05 | P < .05 | |
| 52 | 3 (37*) | 10.5 (37) | 18.8 (37) | 7.6 (35) | 13.5 (37) | 24.5 (36) |
| P = NS | P = NS | P = NS | P < .05 | P < .05 | P < .05 |
Numbers represent the median of the proportion of PNH cells within the subpopulation of cells at the indicated age.
Abbreviations: n, number of mice analyzed; NS, not significant; ND, not done.
P < .05; in comparison to the previous analysis values are statistically significant different (paired Ttest).
Mouse died under anesthesia.
Because of RBC clumping, initial RBC analysis was discarded.
Lymphocyte subset analysis for the majority of mice was started at the age of 16 weeks (B cells and T cells).