Molecular Characteristics of Purified Recombinant G6PD
| . | G6PD Volendam . | G6PD B . |
|---|---|---|
| Specific activity (IU/mg protein) | 36 ± 3 | 210 ± 15 |
| Km glucose 6-P 25°C (μmol/L) | 211 ± 16 | 65 ± 4 |
| Km NADP 25°C (μmol/L) | 30 ± 3 | 12 ± 2 |
| Ki NADPH 25°C (μmol/L) | 20 | 14 ± 3 |
| Substrate analog utilization | ||
| 2-deoxy-glucose 6-P (%) | 1.5 | 4.0 |
| Galactose 6-P (%) | 1.1 | 8.0 |
| Deamino-NADP (%) | 14 | 59 |
| Electrophoretic mobility (%) | 96 | 100 |
| Heat stability at 51°C | Strongly decreased | Normal |
| . | G6PD Volendam . | G6PD B . |
|---|---|---|
| Specific activity (IU/mg protein) | 36 ± 3 | 210 ± 15 |
| Km glucose 6-P 25°C (μmol/L) | 211 ± 16 | 65 ± 4 |
| Km NADP 25°C (μmol/L) | 30 ± 3 | 12 ± 2 |
| Ki NADPH 25°C (μmol/L) | 20 | 14 ± 3 |
| Substrate analog utilization | ||
| 2-deoxy-glucose 6-P (%) | 1.5 | 4.0 |
| Galactose 6-P (%) | 1.1 | 8.0 |
| Deamino-NADP (%) | 14 | 59 |
| Electrophoretic mobility (%) | 96 | 100 |
| Heat stability at 51°C | Strongly decreased | Normal |
Values are means ± SD of 3 independent duplicate measurements.