Molecular Characteristics of Partially Purified G6PD From Skin Fibroblasts
| . | Patient . | Normal . |
|---|---|---|
| Specific activity (U/g protein) | 49 | 369 |
| Km glucose 6-P 37°C (μmol/L) | 328 | 60 |
| Km NADP 37°C (μmol/L) | 51 | 14 |
| Ki NADPH 25°C (μmol/L) | 33 | 40 |
| Substrate analog utilization | ||
| 2-deoxy-glucose 6-P (%) | 4.4 | <4 |
| Galactose 6-P (%) | 1.9 | 2.4 |
| Deamino-NADP (%) | 20.8 | 60 |
| NAD (%) | 0 | 0 |
| Heat stability at 50°C | Slightly decreased | Normal |
| . | Patient . | Normal . |
|---|---|---|
| Specific activity (U/g protein) | 49 | 369 |
| Km glucose 6-P 37°C (μmol/L) | 328 | 60 |
| Km NADP 37°C (μmol/L) | 51 | 14 |
| Ki NADPH 25°C (μmol/L) | 33 | 40 |
| Substrate analog utilization | ||
| 2-deoxy-glucose 6-P (%) | 4.4 | <4 |
| Galactose 6-P (%) | 1.9 | 2.4 |
| Deamino-NADP (%) | 20.8 | 60 |
| NAD (%) | 0 | 0 |
| Heat stability at 50°C | Slightly decreased | Normal |
Mean values of 2 measurements performed in duplicate on 2 different days. The mean values of the 2 duplicates did not differ by >5%.