Table 3. Comparative Expression of... | American Society of Hematology

Table 3.

Comparative Expression of CD45RA by CD48⁺(Normal) and CD48⁻ (GPI-Deficient) Components of CD4⁺ and CD8⁺ T-Cell Subpopulations in Patients With PNH

Patient ID	CD4⁺Lymphocytes^3-150			CD8⁺ Lymphocytes^3-150
Patient ID	Size of PNH Clone %	CD48⁺CD45RA⁺ %	CD48⁻CD45RA⁺ %	Size of PNH Clone %	CD48⁺CD45RA⁺ %	CD48⁻CD45RA⁺ %
PNH007	24.3	37	83	35.8	89	99
PNH008	23.5	21	71	22.2	47	97
PNH012	21.3	40	89	32.3	95	99
PNH019	10.7	18	67	12.6	67	85
PNH020	16.2	56	83	18.2	44	98
PNH022	7.9	28	74	11.1	58	90
PNH030^3-151	14.0	32	46	4.3	63	59
PNH033	21.7	37	77	20.7	42	93
PNH034	1.4	37	74	3.2	64	89
PNH042	0.7	23	71	0.7	36	85
PNH043	5.9	45	85	4.4	63	89
PNH047	0.1	33	ND	0.8	42	ND
PNH055	4.3	61	78	8.9	79	96
PNH058	6.1	51	90	7.6	60	88
PNH063	9.6	7	45	10.4	35	73
PNH072	15.9	40	87	26.0	86	99
PNH079	16.7	42	72	20.7	44	88
PNH087	3.7	43	95	2.7	80	97
PNH090	11.8	45	93	16.2	67	89
PNH091	17.5	16	71	14.6	24	53
Median	10.7	37	77.5	12.6	60	89.5
Normal range^3-152		30-52%			54-82%

Patient ID	CD4⁺Lymphocytes^3-150			CD8⁺ Lymphocytes^3-150
Patient ID	Size of PNH Clone %	CD48⁺CD45RA⁺ %	CD48⁻CD45RA⁺ %	Size of PNH Clone %	CD48⁺CD45RA⁺ %	CD48⁻CD45RA⁺ %
PNH007	24.3	37	83	35.8	89	99
PNH008	23.5	21	71	22.2	47	97
PNH012	21.3	40	89	32.3	95	99
PNH019	10.7	18	67	12.6	67	85
PNH020	16.2	56	83	18.2	44	98
PNH022	7.9	28	74	11.1	58	90
PNH030^3-151	14.0	32	46	4.3	63	59
PNH033	21.7	37	77	20.7	42	93
PNH034	1.4	37	74	3.2	64	89
PNH042	0.7	23	71	0.7	36	85
PNH043	5.9	45	85	4.4	63	89
PNH047	0.1	33	ND	0.8	42	ND
PNH055	4.3	61	78	8.9	79	96
PNH058	6.1	51	90	7.6	60	88
PNH063	9.6	7	45	10.4	35	73
PNH072	15.9	40	87	26.0	86	99
PNH079	16.7	42	72	20.7	44	88
PNH087	3.7	43	95	2.7	80	97
PNH090	11.8	45	93	16.2	67	89
PNH091	17.5	16	71	14.6	24	53
Median	10.7	37	77.5	12.6	60	89.5
Normal range^3-152		30-52%			54-82%

As determined by three-color flow cytometry using CD48/CD45RA/CD4 and CD48/CD45RA/CD8 combinations of monoclonal antibodies as described in Materials and Methods section. Values in the table are the percentage of either CD48⁺ or CD48⁻ cells expressing CD45RA.

Abbreviation: ND, too few cells for reliable assessment of CD45RA staining.

F3-150

T-cell subpopulations identified on the basis of strong CD4 or CD8 positivity and low SSC characteristics.

F3-151

Data from this patient excluded from statistical analysis and median value.

F3-152

Normal ranges derived from a study of 12 normal adult controls.

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